This study was planned to evaluate the effect of different dietary lysine regimens on blood biochemistry and immune response in Lakha, Mianwali, Mushki and Peshawari varieties of indigenous Aseel chicken. One-day-old chicks (n=240), 60 from each of the variety were randomly selected, sub-divided equally into three groups (A, B and C) and offered three dietary lysine regimens (L1, L2 and L3). L1 (1.3% lysine) was offered to group A from 0-6^th week and L2 (1.4-1.2% lysine), wherein 1.4% lysine from 0-3^rd week and 1.2% lysine from 4-6^th week was offered to group B. In L3 (1.5-1.3-1.1% lysine), 1.5, 1.3 and 1.1% lysine from 0-2^nd, 3-4^th and 5-6^th week, respectively, was offered to group C. These birds were kept under RCBD having factorial arrangement of 3 (lysine regimens) × 4 (varieties) × 20 (replicates) with one bird in each replicate. After six weeks, 72 birds including 18 from each variety were selected to collect the blood samples for analysis of blood biochemicals and immune response. The collected data were analyzed by ANOVA and treatment means were compared by DMR test. The findings of this study showed that birds fed on L3 lysine regimen had significantly higher (P≤0.05) serum glucose, total protein, globulin, high density lipoprotein, triglycerides and antibody titer against Newcastle disease virus (NDV) and Infectious bronchitis virus (IBV). While, Lakha and Peshawari varieties presented an overall improved picture of their blood biochemical profile and immune response against NDV and IBV.

Key words: Aseel varieties, Blood biochemical profile, Dietary lysine regimens, Immune response, Serum enzymes