The purpose of this study was to determine the effectiveness of organic, inorganic and mixed toxin binders (TB) commonly used in poultry feeds to bind aflatoxin B1 (AFB1) in in vitro conditions. A total of 20 TBs, which were commonly used in the poultry industry, were divided into three groups as organic, inorganic and mix according to their contents. For each TB was used in in vitro poultry mediums both at pH 3 and 6.8 to represent proventriculus and intestine of poultry, respectively. Control groups were prepared from standard AFB1 and its dilutions; and the treatment groups were prepared from TBs (10mg) and standard AFB1 and its dilutions. After mixing and incubation periods, the samples (8 control, 6 organic, 6 inorganic and 8 mix) were analyzed in 4 replicates (2 for pH 3 and 2 for 6.8) by the method of Vicam aflatest method in HPLC-FLD after extraction and filtration processes. The binding capacity of inorganic TBs was found to be the highest, 98 and 93% at pH 3.0 and 6.8 while the organic TBs were the lowest, 40 and 45% at pH 3.0 and 6.8 respectively. Likewise, the binding capacity of mix TBs to AFB1 was found 96 and 88% at pH 3.0 and 6.8 in in vitro conditions. It was concluded that the inorganic and mix TBs were found to be effective to bind AFB1 among the TBs and could be applied to reduce the negative effects of AFB1 in poultry feeds. The organic TBs were found to be not sufficient to bind AFB1.