previous page   Pak Vet J, 2018, 38(2): 159-164   next page
The Role of csgA and bcsA Genes on Biofilm Formation and Virulence in Salmonella enterica Serovar Typhimurium
Muhanad El Hag1,2,3,4,5, Zheng Feng1,2,3, Yangyang Su1,2,3, Tao Qin1,2,3, Sujuan Chen1,2,3, Daxin Peng1,2,3,4 * and Xiufan Liu1,2,3
1College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; 2Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, PR China
3Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease; Yangzhou, Jiangsu, 225009, P.R. China; 4Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education, Yangzhou, Jiangsu, 225009, P.R. China; 5Faculty of Public and Environmental Health, University of Khartoum, Sudan; *Corresponding author:


Biofilm formation can increase bacterial resistance to adverse conditions. However, limited information is available regarding the roles of the csgA and bcsA genes involved in biofilm formation and virulence for Salmonella enterica serovar Typhimurium. Here we deleted the csgA and bcsA genes in S. Typhimurium strains S016 and S025 and assessed several aspects of biofilm formation and virulence. The ΔcsgA strains did not produce curli fimbriae and ΔbcsA mutants had decreased cellulose production. The ΔcsgA strains were unable to form biofilms. The ΔcsgA strains also showed decreased adhesion and invasion to HeLa cells and reduced intracellular proliferation in HD11 macrophages. The ΔbcsA mutants had similar adhesion, invasion, and proliferation as compared to the wild-type strains. The ΔcsgA strains were significantly attenuated in the virulence in assays involving oral challenge of one-day-old chickens. These findings clarify the respective roles of csgA and bcsA in biofilm formation and pathogenicity of S. Typhimurium.

To Cite This Article: El Hag M, Feng Z, Su Y, Qin T, Chen S, Peng D and Liu X, 2018. The role of csgA and bcsA genes on biofilm formation and virulence in Salmonella enterica serovar Typhimurium. Pak Vet J, 38(2): 159-164.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)