In
Vitro
Attachment and Distribution of
Pasteurella multocida B:2 in the Lung and Urinary Bladder of
Buffaloes
Y Puspitasari, S Annas, MN Adza-Rina and M Zamri-Saad*
Research Centre for Ruminant Diseases, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang, Malaysia *Corresponding author: mzamri@upm.edu.my
Abstract
Haemorrhagic septicaemia is caused by
Pasteurella multocida B:2 that leads
to septicaemia following adherence and colonization of the respiratory tract.
However, recent studies revealed the possible involvement of urinary tract in
the pathogenesis of haemorrhagic septicaemia. This study is conducted to
determine the in vitro adherence and
distribution of P. multocida B:2 in
the lungs and urinary bladder of buffaloes. Three buffalo calves with no history
of vaccination against haemorrhagic septicaemia were killed before lung and
bladder explants were prepared. The explants were then challenged with 109
cfu/ml of live P. multocida B:2. At
the same time, a known respiratory tract bacterium,
Mannhemia haemolytica A:2 and a septicaemic
E. coli were used as comparison. The explants were harvested at 2-h
intervals until 12 hours before the rate of adherence was determined using
scanning electron microscopy while the distribution was determined using
immunoperoxidase staining. All bacterial strains showed similar adherence and
distribution patterns. Pasteurella
multocida B:2 showed significantly (P<0.05) increased rates of adherence and
distribution with time to reach peak at 8-10 h and 12 h post-inoculation,
respectively. There were significant (P<0.05) correlation between the rate of
adherence and distribution. In general, M.
haemolytica A:2 remained least septicaemic bacterium with relatively low
rates of adherence and distribution while
E. coli was the most septicaemic with highest rates of adherence and
distribution.
To Cite This Article:
Puspitasari Y, Annas S, Adza-Rina MN and Zamri-Saad M, 2018. In vitro
attachment and distribution of
Pasteurella multocida B:2 in the lung and urinary bladder of buffaloes. Pak
Vet J, 38(4): 414-418. http://dx.doi.org/10.29261/pakvetj/2018.077