Optimization of Cardioprotective Potential of
Various Concentrations of Medicinal Plants by Using Response Surface
Methodology
Nadia Afsheen1, Khalil-ur-Rehman1,
Nazish Jahan2*, Khalid Mahmood Khan1 and
Muhammad Anjum Zia1
1Department
of Biochemistry; 2Department of Chemistry, University of
Agriculture, Faisalabad, Pakistan *Corresponding author:
nazishjahanuaf@yahoo.com
Abstract
This study was aimed to optimize the various
concentrations of selected medicinal plants through Response Surface Methodology
(RSM) in conjunction with Central Composite Design (CCD) to assess their
therapeutic doses for cardioprotection. Dose response relation is an important
tool to study pharmacological efficacy and therapeutic index of herbal
medicines. In this study the toxicological assay of various concentrations of
Rauvolfia serpentina, Eletaria cardamom, Coriandrum
sativum, Piper nigrum, Allium sativum,
Crataegus oxyacantha and Terminalia
arjuna was performed prior to in vivo
evaluation. The toxicological findings depicted that none of the selected
medicinal plant showed any toxicity, therefore is declared to be safe for
various cardiovascular disease. Instead of
in vivo trial of hundreds of the possible doses, RSM
suggested only five doses (80, 110, 140, 170 and 200 mg/kg b.wt) to explore
cardioprotective potential of selected medicinal plants
in rats. Blood samples were
taken at different time intervals to analyze the cardiac markers (CK-MB, LDH and
SGOT). These cardiac markers were statistically analyzed by “RSM” to get the
optimal therapeutic dose of each
selected medicinal plants. The results revealed that the R. serpentina,
C. oxyacantha,T. arjuna, E.
cardamom, C. sativum,
P. nigrum and A. sativum showed maximum cardioprotection at corresponding
concentration of 164, 172, 165, 190, 183, 186 and 170 mg/kg b.wt.
To Cite This Article:
Afsheen N, Rehman KU, Jahan N, Khan KM and Zia
MA, 2019. Optimization of cardioprotective potential of various concentrations
of medicinal plants by using response surface methodology. Pak Vet J, 39(1):
13-18. http://dx.doi.org/10.29261/pakvetj/2018.111