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Molecular Typing of Brucella abortus Strains Isolated from Cattle in Different Districts of Pakistan Based on Bruce-Ladder-PCR and MLVA-16 Assays
Shahzad Ali1*, Shamim Akhter2, Iahtasham Khan3, Haroon Ahmed4, Abdul Maalik1, Heinrich Neubauer5, Falk Melzer5 and Hosny El-Adawy5,6
1Wildlife Epidemiology and Molecular Microbiology Laboratory (One Health Research Group), Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan; 2Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan; 3Department of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, Pakistan; 4Department of Biosciences, COMSATS Institute of Information Technology (CIIT); Park Road, Chakh Shahzad, Islamabad, Pakistan; 5Institute of Bacterial Infection and Zoonoses, Friedrich-Loeffler-Institut, Jena, Germany; 6Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, 35516, Egypt
*Corresponding author:


Bovine brucellosis is endemic in Pakistan. But little is known about the prevalent Brucella species and biovars. We have performed molecular typing of 17 Brucella isolates from cattle by Bruce-Ladder-PCR and Multiple-Locus Variable number tandem repeat Analysis (MLVA)-16 genotyping. These Brucella isolates were recovered from aborted fetuses, milk samples and vaginal swabs from recently aborted cattle in different districts in Pakistan. All isolates were confirmed by conventional culture methods and PCR. All strains had identical MLVA-16 profiles. Results of this study concluded that the same genotype of B. abortus is circulating in different districts of Pakistan which considered as the main risk of spread of this infection in Pakistan. This data will be helpful to develop prevention and control strategies for human and bovine brucellosis in Pakistan.

To Cite This Article: Ali S, Akhter S, Khan I, Ahmed H, Maalik A, Neubauer H, Melzer F, El-Adawy HH, 2019. Molecular typing of Brucella abortus isolated from cattle in different districts of Pakistan based on Bruce-ladder-PCR and MLVA-16 assays. Pak Vet J, 39(3): 463-465.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)