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Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract through Downregulating CA2 Gene and Enhancing Liver Functions
Hammad Qamar1,2, Khalid Mehmood2,3, Aoyun Li2, Muhammad Waqas2,4, Mudassar Iqbal2,3, Lihong Zhang2, Xiaole Tong2 and Jiakui Li1,2,*
1College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China; 2College of Animals Husbandry and Veterinary Medicine, Tibet Agricultural and Animal Husbandry University, Linzhi, Tibet 860000, PR China; 3University College of Veterinary & Animal Sciences, Islamia University of Bahawalpur-63100, Punjab, Pakistan; 4Faculty of Veterinary & Animal Sciences, University of the Poonch, Rawalakot, District Poonch 12350, Azad Jammu & Kashmir, Pakistan
*Corresponding author:


Globally Tibial Dyschondroplasia (TD) is a serious metabolic syndrome in broilers that is identified by the accretion of the growth plate (GP) into the tibialmetaphyseal portion. Grape seed extract (GSE) is a well-known significant antioxidant since it possesses multifaceted anti-inflammatory, anti-cancer and reducing reactive oxygen ability. The experiment was intended to evaluate the proficiency of GSE to recover the chicks affected with TD. For this purpose, 210 chicks were bought and fed normal starter feed for the first 3 days. After that two groups were made having 70 (Control group; normal feed) and 140 chickens (Thiram group; normal feed+ thiram @ 50 mg/kg). Further two equal divisions were made in Thiram group on 7th day viz TD group and GSE group (@ 50 mg/kg/day). GSE significantly (P<0.05) reverted the blood serum profile and liver antioxidants metabolites and, reduced the histopathological abrasion of the liver as compared to the TD group. Furthermore, GSE chicks exhibited a significant decrease (P<0.05) in CA2 gene expression and less mortality, TD score and GP size. In inference, GSE is a natural plant-based extract which owns solid scavenging and bone healing property, and strong antioxidant capacity. GSE provided recovery to chicks affected with TD chicks via dropping TD score, whereas stabilized the GP width through the CA2 mRNA downregulation.

To Cite This Article: Qamar H, Mehmood K, Li A, Waqas M, Iqbal M, Zhang L, Tong X and Li J, 2019. Recovery of chickens affected with tibial dyschondroplasia by application of grape seed extract through downregulating ca2 gene and enhancing liver functions. Pak Vet J, 39(4): 527-533.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)