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Comparative Association of slc11a1 (Nramp1) Gene with Susceptibility and Resistance to Brucellosis in Various Cattle and Buffaloes Breeds of Pakistan

Raheela Akhtar1, Muhammad Zaheer Iqbal1, Azmat Ullah1, Muhammad Muddassir Ali2*, Beenish Zahid3, Muhammad Ijaz4, Arif Ullah2 and Muhammad Sulman Ali Taseer3

1Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan
2Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan
3Khan Bahadar Chaudhry Mushtaq Ahamd College of Veterinary and Animal Sciences Narowal, Sub-Campus   University of Veterinary and Animal Sciences, Lahore, Pakistan
4Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore, Pakistan
*Corresponding author:


It is observed that different Nramp1 genotypes are linked with Brucella resistance or susceptibility. So, current study aimed to find the Brucella resistance and susceptibility of local and exotic breeds of Pakistan on the basis of Nramp1 genotypes. Two breeds of buffaloes and five breeds of cattle were screened for brucellosis. The overall seropositivity determined by RBPT in cattle and buffaloes was 77.5% and 33.5% respectively. PCR results showed 54.5 and 25% brucella positive cases in cattle and buffaloes respectively. Nili-Ravi buffaloes pertain resistant genotype Nramp1 BB as compared to Kundi breeds of buffaloes possessed susceptible genotype Nramp1 AA. Similarly, Sahiwal breed was more resistant for brucellosis compared to cross breeds and Friesian. Phylogenetic analysis showed that Nramp1 gene of local breed had more closeness with B. taurus of China. This gene resistance can be used for genomic selection of animals to reduce disease cost and economic losses due to brucellosis.

To Cite This Article: Akhtar R, Iqbal MZ, Ullah A, Ali MM, Zahid B, Ijaz M, Ullah A and Taseer MSA, 2019. Comparative association of slc11a1 (Nramp1) gene with susceptibility and resistance to brucellosis in various cattle and buffaloes breeds of Pakistan. Pak Vet J, 39(4): 612-614.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)