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Whole Yeast Expressing Recombinant Fiber 2 Protein Vaccine Candidate Protects Chicken against Fowl Adenovirus Serotype 4 Infection

Van Dam Lai1, Joo-Sung Son1, Tea-Sik Kim1, Kang-San Kim1, Eui-Sung Choi2 and In-Pil Mo1*

1Avian Disease Laboratory, College of Veterinary Medicine, Chungbuk National University, Cheongju, 361-763, South Korea; 2Biotechnology Process Engineering Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, South Korea; *Corresponding author:


Fowl adenoviruses (FAdVs) are prevalent infectious agents in a variety of avian species including chickens worldwide. Among several serotypes, fowl adenovirus serotype 4 (FAdV-4) is known to cause mass economic damages to chicken farms in Korea as a pathogen for the hepatitis-hydropericardium syndrome (HHS). The encoding portions of hexon and fiber genes of FAdV-4 field strain ADL15 0401 were fully sequenced and three constructs, Hexon-L1-His and two constructs of fiber genes Fiber-1-His and Fiber-2-His were ligated with the pYEGa-HIR525α which was optimized for producing complex proteins in Saccharomyces Cerevisiae yeast systems. The yeast cell containing these recombinant proteins were inoculated in specific pathogen-free (SPF) chickens. Antibodies against FAdVs were identified from all immunized chicken groups by commercial ELISA as early as 1 week post inoculation and better virus neutralization in Fiber-2 group with 2 logs neutralizing ability. Finally, the protective efficacy result showed the full protection after challenge with virulent FadV-4 virus in the SPF chicken from Fiber-1 and Fiber-2 groups. These results suggest that the whole yeast expressing recombinant fiber-2 protein can be a candidate for vaccines against the FAdV-4 infection.

To Cite This Article: Lai VD, Son JS, Kim TS, Kim KS, Choi ES and Mo IP, 2020. Whole yeast expressing recombinant fiber 2 protein vaccine candidate protects chicken against Fowl adenovirus serotype 4 infection. Pak Vet J, 40(1): 49-54.  


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)