previous page   Pak Vet J, 2020, 40(3): 394-396   next page
Single Nucleotide Polymorphisms in the Promoter of CD4 Gene are Associated with Production and Mastitis Traits in Dairy Cattle
Arsalan Rasheed, Nawab Ali, Sadaf Niaz1, Saqib Zeb1, Irfan Khattak2, Adnan Khan3, Iftikhar Ahmad4, Yachun Wang3* and Tahir Usman2*
1Departmentof Zoology, Abdul Wali Khan University Mardan 23200, Pakistan; 2College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan 23200, Pakistan; 3Key Laboratory of Agricultural Animal Genetics and Breeding, College of Animal Sciences and Technology, China Agriculture University Beijing 100193, PR China; 4Department of Livestock Management, The University of Agriculture Peshawar 25000, Pakistan
§These authors contributed equally to this work
*Corresponding author:,;


The present study aimed at investigating the effects of single nucleotide polymorphisms (SNPs) in the promoter of CD4 gene with mastitis and production traits in dairy cattle. Blood and milk samples were collected from 201 lactating dairy cattle maintained at government dairy farms in the Khyber Pakhtunkhwa, Pakistan. SNPs were identification in the pool of 30 randomly selected DNA samples followed by genotyping of these SNPs in all samples and statistical analysis by the general linear model of SAS (9.4). Two SNPs (SNP1 104010804G>A and SNP2 104010868T>C) were found associated significantly with the annual milk yield and the frequency of mastitis (P<0.05). The AA genotype of SNP1 and the TT genotype of SNP2 were significantly associated with milk yield and frequency of mastitis compared to other genotypes (P<0.05). Thus, the genotypes significantly associated with mastitis and production traits could be used as significant molecular marker in dairy cattle breeding programs.

To Cite This Article: Rasheed A, Ali N, Niaz S, Zeb S, Khattak I, Khan A, Ahmad I, Wang Y and Usman T, 2020. Single nucleotide polymorphisms in the promoter of CD4 gene are associated with production and mastitis traits in dairy cattle. Pak Vet J, 40(3): 394-396.  


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)