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Clinico-Hemato-Biochemical and Molecular Diagnostic Investigations of Peste des Petits Ruminants in Goats

Syed Abdul Khaliq1§, Mudassar Mohiuddin2,3§, Mudasser Habib4, Riaz Hussain2, Muhammad Abbas1, Xiaoxia Du5, Azam Ali Nasir6, Ayesha Mohi ud Din7, Ahrar Khan5,8* and Jiang Bayi5**

1Livestock and Dairy Development Department, Government of Punjab, Pakistan; 2Department of Pathobiology, University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan; 3Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, Guangdong, China; 4Animal Sciences Division, Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan; 5Shandong Vocational Animal Science and Veterinary College, Weifang, 261061, China; 6Veterinary Research Institute, Lahore, Pakistan; 7Department of Biotechnology, Virtual University of Pakistan, Lahore, Pakistan; 8Faculty of Veterinary Science, University of Agriculture, Faisalabad-38040, Pakistan
*Corresponding authors: (AK)*; (JBY)**


Peste des petits ruminants (PPR) is an acute, infectious and devastating disease of small ruminants, especially for goats. Recently, an outbreak of PPR occurred at a goat farm in Nankana District, Punjab province, Pakistan with 100% (n=105) morbidity and 24% (25/105) mortality. The goats showed characteristic signs of PPR including high temperature, oculo-nasal discharges, diarrhea and ulcerative lesions in the oral cavity. The clinical signs, pathological lesions, hematological values, and serum biochemistry were studied. On postmortem examination, severe pneumonia and enteritis were observed in infected animals. There was marked lymphopenia, decreased erythrocytes level with increased mean corpuscular hemoglobin volume (MCV). The release of albumin and pus cells in urine indicated the kidney damage. Clinical outcome, gross lesions, and histopathological findings were suggestive of Peste des petits ruminants virus (PPRV) infection, which was confirmed by the application of antigenically conserved N gene-based RT-PCR. More than 70% of clinically infected animals were found positive for PPR virus (PPRV) using the RT-PCR. Further investigations carried out to understand the phylogenetic relationship revealed lineage IV PPR viruses involved in the outbreak having more than 90% similarity with isolates previously reported from Pakistan. Pakistan is still in the endemic state for PPR as various outbreaks have been reported from various regions of the country. Regular monitoring of PPR disease and viruses spread are essential for the implementation of appropriate control actions and to know the risk assessment.

To Cite This Article: Khaliq SA, Mohiuddin M, Habib M, Hussain R, Abbas M, Du X, Nasir AA, Din AMU, Khan A and Bayi J, 2020. Clinico-hemato-biochemical and molecular diagnostic investigations of Peste des petits ruminants in goats. Pak Vet J, 40(3): 313-318. 


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)