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Serological Study for the Detection of Antibodies against Leptospira in Goats

Muhammad Umair Aziz1, Muhammad Ijaz1*, Arslan Ahmed1, Awais Ghaffar1, Hammad Nayyar Ghauri1, Muhammad Zeeshan Zafar2, Muhammad Altaf1, Farah Nadia Sheikh3, Imtiaz Ahmad4 and Waseem Shahzad5

1Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore (54600), Pakistan
2Department of Microbiology, University of Veterinary and Animal Sciences, Lahore (54600), Pakistan; 3Medicine department, Sir, Ganga Ram Hospital Lahore (54000), Pakistan; 4Department of Veterinary Clinical Sciences, The University of Poonch Rawalakot Azad Kashmir, Pakistan; 5Veterinary Research Institute-Lahore (54000), Pakistan
*Corresponding author:


Leptospirosis is globally distributed disease of zoonotic significance, caused by pathogenic spirochetes of the genus Leptospira. There is no study regarding the sero-epidemiological investigation of leptospirosis in goats of Pakistan. The study was intended to assess the sero-prevalence and risk factors of Leptospira along with the alterations in hemato-biochemical parameters between seropositive and seronegative goats. Serum samples from 155 goats were collected aseptically from tehsil Pattoki and screened by indirect ELISA to detect anti-Leptospiral antibodies. A total of 15 herds were selected and from each herd 10-15 goats were taken for sampling by convenient sampling technique. The records regarding hypothesized risk factors were analyzed by logistic regression model on SPSS. The overall sero-reactivity to Leptospira was 21.29%. The potential risk factors contributing towards disease occurrence were; age of animals, different types of rearing systems, grazing status, call of veterinary professionals and body condition scores of animals. Hematological parameters of seropositive animals showed significant variations (p<0.05) in values of total erythrocyte count (TEC), total leukocyte count (TLC), hemoglobin (Hb) and packed cell volume (PCV) as compared to seronegative animals. The serum biochemical values for alanine transaminase (ALT) and blood urea nitrogen (BUN) in seropositive goats increased significantly (p<0.05). The study emphasizes comprehensive control strategies to minimize the losses associated with the occurrence of this zoonotic malaise.

To Cite This Article: Aziz MU, Ijaz M, Ahmed A, Ghaffar A, Ghauri HN, Zafar MZ, Altaf M, Sheikh FN, Ahmad I and Shahzad W, 2020. Serological study for the detection of antibodies against Leptospira in goats. Pak Vet J, 40(3): 380-384.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)