High fat high sugar (HFHS) diet results in various disorders including oxidative stress. In present study, prebiotics supplementation was given to rats following HFHS diet feeding. The results showed that prebiotics significantly lowered the HFHS-diet associated elevated levels of cholesterol, triglyceride, low density lipids, alkaline phosphatase, blood urea, creatinine, uric acid and total proteins. Prebiotics significantly restored the HFHS-diet induced decrease in total anti-oxidant capacity. The levels of alanine aminotransferase, aspartate aminotransferase, bilirubin, total oxidation status, malondialdehyde, paraoxonase and arylesterase were not significantly different in HFHS-Prebiotics group as compared to control group. Histological analyses of liver, intestine and kidney tissues in HFHS-group showed cytoplasmic vacuolation, mucosal damage, hepatic triad abnormalities, eccentric nuclei, focal necrosis, tubular congestion and neutrophil infiltration which were significantly improved in HFHS+Prebiotics group suggesting ameliorative potential of prebiotics. In conclusion, our results demonstrated that prebiotics possess therapeutic potential in ameliorating HFHS-diet associated alterations in metabolic profile, oxidative stress markers and histological architecture in intestine, liver and kidney tissues.

To Cite This Article: Rashid H, Hussain Z, Ishtiaq SM, Rasheed MU, Faisal MN, Aslam B, Muhammad F, Babar W, Abbas RZ and Khan JA, 2020. Prebiotics supplementation ameliorates high fat high sugar diet-associated oxidative stress. Pak Vet J, 40(4): 467-473. http://dx.doi.org/10.29261/pakvetj/2020.062