Methanolic Extract of
Fraxinus xanthoxyloides Attenuates Cisplatin-induced Reproductive
Toxicity in Male Albino Rats
Muhammad Umar Ijaz1,
Muhammad Azam Khan1, Saima Yousaf1*, Shabab
Nasir2, Huma Naz3, Haseeb Anwar4,
Tahira Younis2 and Abdul Samad1
1Department
of Zoology, Wildlife and Fisheries, University of Agriculture,
Faisalabad, Pakistan: 2Department of Zoology, Government
College University, Faisalabad, Pakistan: 3Department of
Zoology, Cholistan University of Veterinary and Animal Sciences,
Bahawalpur: 4Department of Physiology, Government College
University, Faisalabad, Pakistan
*Corresponding author:
s.yousaf575@gmail.com
Abstract
Cisplatin is the most
effective chemotherapeutic drug used to treat the several types of tumors.
Fraxinus xanthoxyloides is a plant
with several pharmacological and biological activities. This research was aimed
to find the curative potential of F.
xanthoxyloides extract to counter the cisplatin-induced testicular damage in
albino rats. Male albino rats were used for this trial. Rats were distributed
into eight equal groups. Group I: control, Group II: vehicle control; Group III:
cisplatin (10 mg/kg i.p.); Group IV: cisplatin (10 mg/kg) and silymarin (100
mg/kg); Group V: cisplatin (10 mg/kg) and
F. xanthoxyloides (200 mg/kg); Group VI: cisplatin (10 mg/kg) and
F. xanthoxyloides (400 mg/kg); Group
VII: F. xanthoxyloides (200 mg/kg) and
Group VIII: F. xanthoxyloides (400
mg/kg). Rats were slaughtered on 46th day of the experiment and
testes and blood samples were collected. Hormonal analysis, antioxidant enzymes
and histopathological changes were assessed. Cisplatin treatment induced
significant (P<0.05) reduction in LH, FSH and testosterone concentrations.
Activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and
glutathione reductase (GSR) were significantly (P<0.05) reduced while
significant (P<0.05) increase in the thiobarbituric acid reactive substances
(TBARS) level was observed. Cisplatin significantly (P<0.05) decreased the
seminiferous tubules diameter, tunica albuginea height, epithelial height,
spermatogonia, primary and secondary spermatocytes and spermatids while
significantly (P<0.05) increased the interstitial spaces and tubular luminal
diameter. Whereas F. xanthoxyloides
restored all these damages and abnormalities to their normal level. It is
concluded that F. xanthoxyloides
extract have capability to ameliorate the cisplatin-induced testicular toxicity
in male albino rats.
To Cite This Article: Ijaz MU, Khan MA,
Yousaf S, Nasir S, Naz H, Anwar H, Younis T and Samad A, 2020. Methanolic extract of
Fraxinus xanthoxyloides attenuates cisplatin-induced reproductive
toxicity in male albino rats. Pak Vet J, 40(4):
489-493. http://dx.doi.org/10.29261/pakvetj/2020.048