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Methanolic Extract of Fraxinus xanthoxyloides Attenuates Cisplatin-induced Reproductive Toxicity in Male Albino Rats
Muhammad Umar Ijaz1, Muhammad Azam Khan1, Saima Yousaf1*, Shabab Nasir2, Huma Naz3, Haseeb Anwar4, Tahira Younis2 and Abdul Samad1
1Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan: 2Department of Zoology, Government College University, Faisalabad, Pakistan: 3Department of Zoology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur: 4Department of Physiology, Government College University, Faisalabad, Pakistan
*Corresponding author:


Cisplatin is the most effective chemotherapeutic drug used to treat the several types of tumors. Fraxinus xanthoxyloides is a plant with several pharmacological and biological activities. This research was aimed to find the curative potential of F. xanthoxyloides extract to counter the cisplatin-induced testicular damage in albino rats. Male albino rats were used for this trial. Rats were distributed into eight equal groups. Group I: control, Group II: vehicle control; Group III: cisplatin (10 mg/kg i.p.); Group IV: cisplatin (10 mg/kg) and silymarin (100 mg/kg); Group V: cisplatin (10 mg/kg) and F. xanthoxyloides (200 mg/kg); Group VI: cisplatin (10 mg/kg) and F. xanthoxyloides (400 mg/kg); Group VII: F. xanthoxyloides (200 mg/kg) and Group VIII: F. xanthoxyloides (400 mg/kg). Rats were slaughtered on 46th day of the experiment and testes and blood samples were collected. Hormonal analysis, antioxidant enzymes and histopathological changes were assessed. Cisplatin treatment induced significant (P<0.05) reduction in LH, FSH and testosterone concentrations. Activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and glutathione reductase (GSR) were significantly (P<0.05) reduced while significant (P<0.05) increase in the thiobarbituric acid reactive substances (TBARS) level was observed. Cisplatin significantly (P<0.05) decreased the seminiferous tubules diameter, tunica albuginea height, epithelial height, spermatogonia, primary and secondary spermatocytes and spermatids while significantly (P<0.05) increased the interstitial spaces and tubular luminal diameter. Whereas F. xanthoxyloides restored all these damages and abnormalities to their normal level. It is concluded that F. xanthoxyloides extract have capability to ameliorate the cisplatin-induced testicular toxicity in male albino rats.

To Cite This Article: Ijaz MU, Khan MA, Yousaf S, Nasir S, Naz H, Anwar H, Younis T and Samad A, 2020. Methanolic extract of Fraxinus xanthoxyloides attenuates cisplatin-induced reproductive toxicity in male albino rats. Pak Vet J, 40(4): 489-493.


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