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Rapid Detection of Biofilm Formation by Zoonotic Serovars of Salmonella enterica and Avian Pathogenic E. coli Isolates from Poultry
Shiza Nawaz1,2, Muhammad Moman Khan2, Jonas Noack2, Asad Bashir Awan1, Juliane Schiebel2, Dirk Roggenbuck2,3, Peter Schierack2, Yasra Sarwar1 and Aamir Ali1*
1National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College of Pakistan Institute of Engineering and Applied Sciences (PIEAS), Faisalabad, Pakistan, 2Institute for Biotechnology, Brandenburg University of Technology (BTU) Cottbus-Senftenberg, Senftenberg, Germany, 3Faculty of Health Sciences, Joint Faculty of the Brandenburg University of Technology Cottbus – Senftenberg, the Brandenburg Medical School Theodor Fontane and the University of Potsdam, Germany
*Corresponding author:


Biofilms are complex, sessile microbial communities that are problematic in clinical settings due to their association with survival and pathogenicity of bacteria. The biofilm formation supporting conditions for zoonotic serovars of Salmonella and avian pathogenic E. coli (APEC) from poultry have not been well studied yet. Clinical isolates of zoonotic Salmonella and APEC from poultry were evaluated for biofilm formation in four media at 37°C and 40°C after incubation of 48 and 72 hrs. The biofilms formed in 96 well plates were visualized and quantified with a new module of Aklides system using fluorescence microscope coupled with automated VideoScan Technology. After 72 hrs, brain heart infusion at 40°C and Rappaport-Vassiliadis Soya broth at 37°C were found most suitable for APEC and Salmonella biofilm formations respectively. The new information will be useful for further biofilm associated studies particularly for evaluation of antibiofilm compounds and contribute in infection control.

To Cite This Article: Nawaz S, Khan MM, Noack J, Awan AB, Schiebel J, Roggenbuck D, Schierack P, Sarwar Y and Ali A, 2020. Rapid detection of biofilm formation by zoonotic serovars of Salmonella enterica and avian pathogenic E. coli isolates from poultry. Pak Vet J, 40(4): 527-530.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)