The antibiotic resistance is creating a serious challenge to treat diseases especially against multidrug resistant (MDR) E. coli. The present study was conducted to evaluate the antibacterial activity of seven different flowers ethanolic extracts named as Achillea millefolium, Bombax ceiba, Chrysanthemum cinerarifolium, Hyssopus officinalis, Rosa damascena Miller, Taraxacum officinale Weber and Woodfordia fruticosa against MDR E. coli through spot test and minimum inhibitory concentrations (MIC) test. The results showed all the seven extracts have significant antibacterial activity against MDR E. coli and control. The MIC of Rosa damascena Miller, Bombax ceiba was 3.125 mg/ml and Taraxacum officinale Weber was 12.5 mg/ml for MDR E. coli. Similarly, Achillea millefolium, Hyssopus officinalis, Chrysanthemum cinerarifolium MIC value was 25 mg/ml and 12.5 mg/ml for Woodfordia fruticosa Kurtz. The current study reveals that these flowers possess strong antibacterial activity and thus can be used as potential antimicrobial agent against various resistant bacterial pathogens.