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Serosurvey and Potential Risk Factors of Brucellosis in Dairy Cattle in Peri-Urban Production System in Punjab, Pakistan
Iahtasham Khan1**, Shahzad Ali2, Riaz Hussain3*, Ahmad Raza1, Muhammad Younus4, Nasrullah Khan5 and Mehwish Faheem6

1Section of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, Sub-Campus Jhang, Pakistan; 2Wildlife Epidemiology and Molecular Microbiology Laboratory (One Health Research Group), Discipline of Zoology, Department of Wildlife & Ecology, University of Veterinary and Animal Sciences, Lahore, Ravi Campus, Pattoki, Pakistan; 3Department of Pathology, Faculty of Veterinary and Animal Sciences, Islamia University Bahawalpur 63100; 4Department of Pathology, KBCMA, College of Veterinary of Veterinary and Animal Sciences, Narowal, Pakistan; 5Department of Social Sciences, University of Veterinary and Animal Sciences, Lahore, Sub-Campus Jhang, Pakistan; Department of Zoology, Government College University Lahore, Pakistan
*Corresponding authors:;


Brucellosis is a well-known contagious and zoonotic infection of different animals which influences the health status of animal and economy of underdeveloped/ developing countries. Therefore, this study was conducted to estimate the sero-prevalence and risk factors of Brucella infection in dairy cattle through reliable diagnostic techniques. A total of 300 cattle was included for blood sampling kept at peri-urban areas of Sargodha, Sahiwal and Chiniot districts of Punjab. All the serum samples were tested through RBPT antigen, i-ELISA and RT-PCR. Results showed that 12.66% animals were seropositive for Brucella infection and all seropositive samples were positive for Brucella genus specific RT-PCR. Abortion history, gender, repeat breeding, lactating animals and pregnancy status were key factors associated with seropositivity of animals. Results on logistic regression analysis indicated significant association of different potential risk factors such as history of abortion, repeat breeding and lactating stage with brucellosis in animals. From the results of this study, it is suggested that there is a need of continuous screening of dairy animals to control brucellosis and to minimize the risk of human brucellosis.

To Cite This Article: Khan I, Ali S, Hussain R, Raza A, Younus M, Khan N and Faheem M, 2021. Serosurvey and potential risk factors of brucellosis in dairy cattle in peri-urban production system in Punjab, Pakistan. Pak Vet J, 41(3): 459-462.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)