1State
Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary
Research Institute, Chinese Academy of Agricultural Sciences,
Xujiaping 1, Lanzhou, 730046, Gansu, People's Republic of China;
2Al-Azhar University, Faculty of science, Assuit 71524,
Egypt
*Corresponding author:
chuyuefeng@caas.cn; chenshengli@caas.cn; hussamaskar@azhar.edu.eg
Abstract
Bovine respiratory diseases are widespread and too costly disease impacting
contributes to economically essential diseases like mastitis and pneumonia
worldwide. The present study aimed to explore
the
influence of
M.
bovis
field strain
07801
on the status of
PBMCs in the challenged cattle group and the
immunized one. For this aim, the PBMCs proliferation, apoptosis, and cytokine
profile changes were determined.
In this study,
M.
bovis
strain Mb
07801
and reference strain
PG45
were
used; ten calves (2-3
months old) were arranged into two groups (5 calve each): 1)
PBS-challenged group (with M.
bovis
07801 at
about
1010 CFU/ml), and 2) immunized group (by Inactivated M.
bovis
07801); both groups were treated nasally and intra-tracheal. Blood samples were
obtained from both groups and examined for PBMCs proliferation and apoptosis, as
well as serum cytokine profile, before infection and at day zero and days 7, 14,
21, and 36 post bacterial treatment. The results revealed that M. bovis
strain (07801and
PG45)
antigen increased the proliferative response
of the stimulated
PBMC compared with the unstimulated cells and ConA-alone stimulated. The PBMC
apoptosis showed a non-significant increase in both challenged and immunized
groups compared to the negative and positive control (treated with apoptosis
inducer) groups. Besides, the levels of cytokines profile showed a significant
up-regulation in IFN-γ, IL-4, IL-2, IL-18, TNF-α, IFN-α, IL-6, IL-10 IL-1β, and
IL-13, in both groups, except IL-4 and IL-18 those recorded a down-regulation in
the immunized group at days 7,14, 21, and 36 post-M.
bovis infection. In conclusion,
immunization markedly ameliorated the immune deterioration induced by M.
bovis
strain
07801.
To Cite This Article:
Askar H, Hao H, Yan X, Ma L, Lan S, Liu Y, Chen S
and Chu Y, 2021. Evaluation of the PBMC Proliferation, Apoptosis and Cytokines Profiling in
Cattle Infected with Mycoplasma bovis Strain 07801. Pak Vet J,
41(3): 321-328.
http://dx.doi.org/10.29261/pakvetj/2021.032