Antimicrobial Effect of Essential Oils on
Multidrug-Resistant Salmonella
typhimurium in Chicken Fillets
Alaa Eldin MA Morshdy1, Boshra M Nahla2*,
Saleh Shafik2 and Mohamed A Hussein1
1Food
Control Department, Faculty of Veterinary Medicine, Zagazig
University, Zagazig, 44519, Egypt
2Animal
Health Research Institute, Mansoura Laboratory, Mansoura, 35511,
Egypt
*Corresponding author:dr.boshramagdy@gmail.com
Abstract
Chicken meat, which could be a healthy and
nutritionally food, is regrettably incriminated as a source of
Salmonella typhimurium which has a
great ability to cause human salmonellosis. This study aimed to assess the
prevalence of multidrug-resistant S.
typhimurium in chicken meat and the effects of essential oils on its
viability. A total of 300 chicken meat and its products samples were streaked on
XLD agar plates, which was followed by identification of the isolates based on
biochemical and serological tests. Ten isolates were serotyped as S.
typhimurium then assayed for susceptibility to 14 antimicrobials by the
single diffusion method. Eight isolates (80%) showed multiple antimicrobial
resistance (MAR) for 3 or more antimicrobials with MAR index of 0.4857 in
average. Serotyped S. typhimurium strain with the highest antimicrobial
resistance,confirmed by 16s RNA
sequencing, was selected for studying the effects of thyme, oregano, and lemon
essential oils with concentrations of 0.5, and 1% on its viability after
inoculating into chicken fillets by intensity of 3.0×106 and on
sensory traits of chicken fillets on 2nd, 4th, 6th,
and 8th days of inoculation during cold storage (4ºC). All results
showed a significant reduction of S.
typhimurium counts with highest inhibition obtained using 1% lemon essential
oil. The sensory properties of treated chicken fillets were improved by all used
essential oils, compared to the control samples after 6th day, and 8th
day of the storage period. The samples treated with 0.5, and 1% lemon essential
oil revealed the highest improvement of sensory attributes. This study proved
that the majority of S. typhimurium existingin chicken meat are
Multidrug-resistant and have no negative effect on sensory traits, hence, posing
a public health hazard. Natural essential oils have, also, great antimicrobial
effect on S. typhimurium, thus it could replace chemical antimicrobials.
To Cite This Article:
Morshdy AEMA, Nahla BM, Shafik S and Hussein MA,
2021. Antimicrobial effect of essential
oils on multidrug-resistant Salmonella
typhimurium in chicken fillets. Pak Vet J, 41(4): 545-551. http://dx.doi.org/10.29261/pakvetj/2021.055