The normal descriptive anatomical and histological studies on feline major salivary glands were estimated. We were focusing on clinically valuable glands; parotid, mandibular and sublingual salivary glands, and their ductal pathways by making sialography and papillary cannulation, that can help surgeons in their surgical approach.  The work was carried out on seven cadaver domestic cats of both sexes. Anatomical dissection was performed to separate each gland and its duct. A novel papillary cannulation technique was achieved along with the radiographical study which occurred by injecting the ductal papillae with lead oxide dissolved in turpentine oil. Our findings asserted that the parotid gland was caudal to the mandible with a serous secretory unit. Its papilla was located dorsally in the upper buccal mucosa with a curved bent to prevent its occlusion with food particles. Thus, sialocoele was rarely seen in the parotid gland. The mandibular and sublingual salivary glands were closely connected in one compact membrane that has both serous and mucous endpieces. Their ducts passed together in the intermandibular space till they opened in the sublingual caruncles that are easily obstructed by food remnants. Therefore, they are more susceptible to sialocoele. The zygomatic gland was the deepest, while the molar gland appeared at the mandibular lip, which is composed mainly of mucous adenomeres. Eventually, we could provide in the current study a complete descriptive atlas of the major salivary glands of domestic cats, and we explained why sialocoele occurs more frequently in mandibulo-sublingual glands than in parotid glands.