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Clinico-Pathological, Immunohistochemical and In-Situ TUNEL ASSAY Observations of Canine Breast Tumors
Wei Chen1,§, Pei Liu1,§, Yulan Zhao1, Changming Zhou1, Guyue Li1, Xiaoquan Guo1, Cheng Huang1, Guoliang Hu1, Cong Wu1, Zheng Xu2, Zuwei Yang1, Ahrar Khan3,4,* and Ping Liu1,*

1Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, PR China; 2Department of Mathematics and Statistics Wright State University Dayton, OH, 45435; 3Shandong Vocational Animal Science and Veterinary College, Weifang, PR China; 4Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan
§These authors contributed equally to this study and share first authorship.
*Corresponding author: (AK); (PL)


Mammary cancer is the most prevalent canine tumor and frequently results in death. This study was carried out for the diagnosis of three canine breast tumors based on clinical, histopathological, immunohistochemistry, and in situ TUNEL assay. Firstly, the morphology of tumor cells was examined by hematoxylin and eosin staining. We found two cases of ductal carcinoma characterized by ductal dilatation and one case of solid carcinoma characterized by extensive connective tissue proliferation. Immunohistochemical assay in paraffin-embedded sections immediately followed surgical removal. The results demonstrated that the expression level of Estrogen receptor (ER), progesterone receptor (PR), p53, vascular endothelial growth factor (VEGF), and proliferating cell nuclear antigen (PCNA) proteins were abnormal. Finally, in situ TUNEL showed a significant decrease in apoptotic cells in canine breast tumors (P<0.01). Taken together, clinical, histopathological, immunohistochemistry, and in situ TUNEL assay will provide a better understanding of the pathogenesis of breast tumors in dogs under field conditions.

To Cite This Article: Chen W, Liu P, Zhao Y, Zhou C, Li G, Guo X, Huang C, Hu G, Wu C, Xu Z, Yang Z, Khan A and Liu P, 2022. Clinico-Pathological, immunohistochemical and in-situ TUNEL assay observations of canine breast tumors. Pak Vet J, 42(1): 131-1134.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)