Acesulfame potassium (Ace-K) is one of the commonly used artificial sweeteners, keeping its sweetening property after heating or freezing encouraging its use in various products especially those consumed by children, and encounters a potential hazard for cumulative toxicity, therefore safety evaluation for its long-term exposure is necessary. A total of 90 mature and immature males Sprague Dawley rats was divided into six groups, 5 animals each: G1&G2 control untreated immature and immature rats, G3&G4 (immature and mature rats treated with Ace-k at dose of 15mg/kg. b.w) and G5&G6 (immature and mature rats treated with Ace-k at dose of 90 mg/kg. b.w), All treated rats received Ace-K  via gastric intubation for 6 days per week for 10 weeks, at the end of experimental period blood samples were collected for the determination of serum amylase, lipase, and glycated hemoglobin, in addition, the pancreas was dissected for histopathological evaluation. Results revealed that chronic treatment with Ace-k induced a significant increase in weight gain in younger age treated groups compared with older ones. Pancreatic enzymes showed non-significant differences between control and treated groups at different ages while significant reduction in glycated hemoglobin was detected in older age receiving higher dose compared with other treated groups. Ace-K induced various pancreatic histopathological changes that exhibited dose dependent increase in lesions severity among treated groups. The present data revealed that chronic treatment of rat with Ace-K induced pancreatic injury. In addition, the histological hallmarks of pancreatic pathology induced by Ace-k give attention to the possible carcinogenic potential of Ace-k on pancreatic tissue.