The present study was designed to transform small-sized follicles into medium sized follicles to enhance their developmental competence under the effect of FSH. Thirty Sahiwal cows were randomly assigned to three groups (n=10 each); A) FSH-00mg (control), B) FSH-100mg (F100), and C) FSH-200mg (F200). On a random day of the estrus cycle (-48h), each cow underwent follicular ablation and received an intra-vaginal CIDR device. After 48h (0 hour), each cow received 150µg of D-cloprostenol; while four equivalent doses of FSH (25 mg in F100 group and 50 mg in F200 group) were administered at 12h intervals (0, 12, 24 and 36 hr). Ovarian ultrasonography was performed on daily basis and Ovum Pick-Up (OPU) was conducted after 40h of last FSH treatment in groups B and C, while 76 hrs after D-cloprostenol injection in control group A. The results revealed that the number of medium sized (6≥10 mm) follicles were significantly (P<0.05) increased in FSH treated (F100 & F200) groups than control, while there was non-significant difference between F100 and F200 groups. During IVEP, the blastocysts yield per OPU session was almost double in F100 (2.00±0.69) and F200 (1.80±0.59) groups than the control (0.90±0.37) group. The in-vitro cultured blastocysts were transferred to four synchronized heifers; three heifers were pregnant and gave birth to four healthy calves, including one twin birth. To the best of our knowledge, this is the first report on the birth of genetically tested Sahiwal calves through OPU-IVEP technique in Pakistan, following fertilization of oocytes retrieved from Sahiwal cows with semen from a Sahiwal bull. In conclusion, ovarian stimulation with FSH hormone improves the proportionality of medium-sized follicles during OPU with better oocyte competence in Sahiwal cattle.