Construction and Evaluation of the Toxoplasma gondii
DNA vaccine targeting DEC-205
Rong Chen1§, Jia jia Peng1§,
Muhammad Mohsin1,2,3§, Xiaohang Huang1§,
Xialin Lin1, Liliana Aguilar- Marcelino4,
Zhijian Huang1 and Guangwen Yin1*
1Engineering
Laboratory of Animal Pharmaceuticals and College of Animal Sciences
(College of Bee Science), Fujian Agriculture and Forestry
University, Fuzhou, Fujian Province, 350002, China; 2College
of Life Science, Fujian Agriculture and Forestry University, Fuzhou,
Fujian Province, 350002, China; 3Department of
Parasitology, University of Agriculture, Faisalabad,Pakistan; 4Centro Nacional de Investigacion
Discliplinaria en Salud Animal e Inocuidad, INIFAP, Km 11 Carretera
Federal Cuernavaca, No. 8534, Col. Progreso, Jiutepec, Morelos, C.P.
62550, Mexico
§These
authors contributed equally to this work.
*Corresponding author:
yinguangwen000@sina.com
Abstract
Toxoplasmosis, caused
by Toxoplasma gondii (T.
gondii), remains a significant public health problem. Recently, it has been
shown that the antigens targeting the dendritic cells (DCs) is a promising way
to enhance the immune responses against pathogens and tumors. In this study, we
constructed and evaluated a new DNA vaccine of T. gondii,
based on the major surface antigen
SAG1 of T. gondii and an antibody
single-chain fragment variable (scFv) directed against the mouse DEC 205, an
endocytic receptor expressed on the surface of DCs and other cells. The
constructs were tested in vitro to
express the encoded proteins, then the
cellular and humoral immune responses were analyzed, and the protective efficacy
of the vaccine was evaluated. Our results showed that the plasmid encoding the
fusion of DEC-205 scFv with SAG1 significantly enhanced the SAG1-specific immune
response and DC targeting could elicit the Th1 type immune response, evidenced
by the high level of IFN-γ, IL-2 and IgG2a. Therefore, we showed evidence in
this research that DEC-205 can be exploited in developing an effective
T. gondii vaccine.
To Cite This Article:
Chen R, Peng JJ, Mohsin M, Huang X, Lin X,
Aguilar-Marcelino L, Huang Z and Yin G, 2022. Construction
and evaluation of the Toxoplasma gondii DNA vaccine targeting DEC-205. Pak Vet J, 42(2): 256-260.
http://dx.doi.org/10.29261/pakvetj/2022.020