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Trends in Frequency, Potential Risks and Antibiogram of E. coli Isolated from Semi-Intensive Dairy Systems
Muhammad Ahsan Anwar1, Sidra Aziz2, Khurram Ashfaq1, Amjad Islam Aqib3*, Muhammad Shoaib4,5, Muhammad Aamir Naseer1, Mughees Aizaz Alvi1, Iqra Muzammil6, Zeeshan Ahmad Bhutta1,7, Huma Sattar8, Arslan Saleem9, Tean Zaheer10 and Fakhara Khanum11 and Asif Mahmood12

1Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, 38000- Pakistan; 2Tehsil Head Quarter Hospital, Mankera-30060, Pakistan; 3Department of Medicine, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, 63100 Pakistan; 4Key Laboratory of New Animal Drug Project, Gansu Province/Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs/ Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, 730050 Lanzhou, PR China; 5Institute of Microbiology, Faculty of Life, Social, and Physical Sciences, Gulab Devi Educational Complex, Lahore-54600 Pakistan; 6Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, 54000-Pakistan; 7Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, 28644, Republic of Korea; 8Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan; 9School of Engineering and Design Technische Universität München, Arcisstraße 2180333 München, Germany; 10Department of Parasitology, University of Agriculture, Faisalabad, 38000, Pakistan; 11National Institute of Food Science and Technology, University of Agriculture Faisalabad-38000, Pakistan; 12Veterinary Research Institute Lahore, Punjab, Pakistan
*Corresponding author:


Semi-intensive dairy systems are prevailing in tropical and subtropical countries that need to be probed for public health concern pathogens. The current study was designed to map trends in prevalence of dairy-originated E. coli, associated risk factors, and altering in-vitro drug susceptibility patterns. A total of n=200 subclinical mastitis milk samples were intended to be collected using purposive sampling method from semi-intensive dairy systems for which n=764 dairy animals (n=440 buffaloes, n=324 cattle) were screened. Standard biochemical and microbiological methods coupled with polymerase chain reaction (23sRNA) were applied to identify E. coli from subclinical milk samples. The study, overall, found a 26.18% (200/764) prevalence of subclinical mastitis and 13.50% (27/200) E. coli from subclinical samples. Among assumed risk factors, lack of use of teat dip (OR=8.26, C.I. = 2.73–24.91), higher age groups (OR=17.87, C.I. = 4.42–72.16), parity number >3 (OR=3.68, C.I. = 1.59–8.49), underweight animals (OR=2.89, C.I. = 1.11–7.53), and mid-lactation (OR=14.94, C.I. = 3.04–73.24) were dominant potential risk factors for E. coli infection. Antibiogram showed 42.86 and 21.43% of E. coli isolates resistant to amoxicillin-clavulanate and oxytetracycline, respectively. It was noted that more than 40% (42.86, 60.87, 57.89, 66.86, and 67.86%) of E. coli fall in intermediate susceptible cadre against 62.5% of tested antibiotics. In conclusion, increasing percentages of E. coli, higher number of potential risk factors, and antibiotic susceptibility inclining towards resistance demands stern compliance in anticipated time to avoid any grave situation.

To Cite This Article: Anwar MA, Aziz S, Ashfaq K, Aqib AI, Shoaib M, Naseer MA, Alvi MA, Muzammil I, Bhutta ZA, Sattar H, Saleem A, Zaheer T, Khanum F and Mahmood A, 2022. Trends in frequency, potential risks, and antibiogram of E. coli isolated from semi-intensive dairy systems. Pak Vet J, 42(2): 167-172.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)