previous page   Pak Vet J, 2022, 42(2): 229-235   next page
Effect of Topical Insulin and Ozonized Cream for the Treatment of Full-thickness Dermal Burn Injuries: A Clinical and Histopathological Study in Diabetic Rats
Gül Çıray Akbaş Güngör1, Çağrı Gültekin1, Aysel Kükner2, İlker Etikan3, Meliha Temizel4 and Fatma Eser Özgencil1

1Department of Surgery, Faculty of Veterinary Medicine, Near East University, Nicosia/TRNC; 2Department of Histology, Faculty of Medicine, Near East University, Nicosia/TRNC; 3Department of Biostatistics, Faculty of Medicine, Near East University, Nicosia/TRNC; 4Experimental Animals and Research Center, Near East University, Nicosia/TRNC
*Corresponding author:;


Diabetic wound healing is a major health problem encountered in both human and veterinary medicine. In this study, 30 Wistar albino rats were experimentally induced diabetes having bilateral full-thickness tissue loss and second-degree burns onto the skin and divided into three groups. The effects of a 14-day-wound treatment with NPH insulin cream (G1), ozonized cream (G2), and ozonized cream + NPH insulin cream (G3) was investigated. Photographic evidence of wound healing was measured on 3, 7 and 14 days. On the fourteenth day, the rats were euthanized, and scar tissue samples were collected for histopathological examination. It was observed that topical NPH insulin alone acted as a strong immunomodulator in preventing long-term inflammation that could lead to delayed wound healing. In G2 and G3 groups, the epithelization score was increased, additionally in G3 the fibroblast activation score was  increased as well. In conclusion, a combination of equal amounts of NPH insulin cream and ozonized cream contributed to wound healing by decreasing inflammation score and increasing epithelization and fibroblast activation.

To Cite This Article: Güngör Akbaş GÇ, Gültekin Ç, Kükner A, Etikan İ, Temizel M, and Özgencil FE, 2022. Effect of topical insulin and ozonized cream for the treatment of full-thickness dermal burn injuries: a clinical and histopathological study in diabetic rats. Pak Vet J, 42(2): 229-235.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)