Molecular Detection and Immune-Profiling of Circulating Very
Virulent Infectious Bursal Disease in Broiler Farms in Egypt
Nashwa Hamad¹, Shiem EL-Sherry ²٭,
Sary Abd-Elghaffar¹ and Mohammed Abdul-Rahman¹
¹Department
of pathology, faculty of veterinary medicine, Assiut University,
Assiut 71526, Egypt
²Department
of poultry diseases, faculty of veterinary medicine, Assiut
University, Assiut 71526, Egypt
*Corresponding author:
shiemelsherry@aun.edu.eg
Abstract
In Egypt, several highly pathogenic infectious bursal disease isolates were
reported to break through high levels of maternal immunity in commercial flocks.
This study investigated viral tropism and immune profiling of circulating very
virulent strains in broiler farms.
Samples from 19 suspected outbreaks were collected. RT-PCR was conducted for
genetic differentiation between very virulent strains and other circulating
strains. Immunohistochemical staining applied on
positive samples for
describing the topographic distribution of viral antigens in bursa of Fabricius,
thymus and spleen. Anti-chicken CD3 and CD79A markers were
used to study the immune profiling of B and T cells.
Among 19 flocks, 78.9% came out positive with RT-PCR. 57.9% were very virulent
strains, 15.8% were classical or vaccinal strains, and 5.2% were coinfected.
Viral antigens were most prevalent in bursa, followed by the spleen but not in
thymic tissue. B cell significantly reduced in moderate and severe lesion scored
bursae, while increased number of intra-bursal T cells was observed. In thymic
cells, depletion of lymphocytes was obvious, although it was not associated with
viral detection. Thymic sections revealed significant reduction in T-cells in
moderate and severe lesion scores. However, mild affected tissue showed
significant increase in T cells. In spleen, significant decrease in T and B
cells was observed in moderate and severe scored samples. Very virulent isolates
induced similar pathogenicity to typical UK661 strain. Providing detailed
information on immune
response against circulating strains may increase our understanding about the
ability of the virus to frequently break through vaccination control.
To Cite This Article:
Hamad N, EL-Sherry S, Abd-Elghaffar S, Abdul-Rahman M
2022.
Molecular detection and immune-profiling of circulating very virulent infectious
bursal disease in broiler farms in Egypt.
Pak Vet J, 42(3): 316-321.
http://dx.doi.org/10.29261/pakvetj/2022.044