previous page   Pak Vet J, 2022, 42(3): 300-307   next page
Evaluating the Effectiveness of Multidrug Resistant Staphylococcus aureus Mastitis Vaccines in Dairy Cattle
Rai Khuda Dad1, Muhammad Avais*1, Jawaria Ali Khan1 and Aftab Ahmad Anjum2

1Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore-54000-Pakistan;
Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore-54000-Pakistan
*Corresponding author:


Indiscriminate use of antibiotics to treat Staphylococcus aureus bovine mastitis may impair mammary glands immune function and develop bacterial resistance. Consequently, developing alternative remedies to treat mastitis is imperative. Current study evaluated plain multidrug resistant S. aureus vaccine (PMRSAV), Montanide oil adjuvanted multidrug resistant S. aureus vaccine (MMRSAV), and Aluminum hydroxide adjuvanted multidrug resistant S. aureus vaccine (AMRSAV) in lactating dairy cattle. Serum samples through IHA revealed that MMRSAV provoked maximum antibody titer at day 90 (274.4±0.41) whereas AMRSAV (147±0.46) and PMRSAV (78.8±0.44) produced highest antibody response at day 60. The cumulative mean serum IHA antibody titer was recorded highest for MMRSAV (148.4) than AMRSAV (78.4) and PMSAV (34.1). The peak milk whey antibody titer was seen at day 60 for PMRSAV (9±0.23) and AMRSAV (14.7±0.24) whereas it was observed highest at day 90 in MMRSAV (26.2±0.25). Somatic cell count (SSC: × 105 mL-1) observed decline trend in all the vaccinated cattle until day 90 for PMRSAV (1.449±0.219), day 120 for MMRSAV (1.201±0.097) and AMRSAV (1.327±0.104). During study period, the highest quarter-based incidence was observed in control group (25%) followed by PMRSAV (17.5%), AMRSAV (10%) and MMRSAV (5%) resulting incidence reduction (80%) for MMRSAV followed by AMRSAV (60%) and PMRSAV (30%). Post challenge lowest overall mean somatic cell count was recorded in group vaccinated with MMRSAV (2.323±0.46) followed by AMRSAV (3.006±0.43), PMRSAV (3.759±0.82) and unvaccinated control group D (7.798±1.11). The study concluded that MMRSAV is the most effective in preventing multidrug resistant S. aureus mastitis in dairy cattle.

To Cite This Article: Dad RK, Avais M, Khan JA, Anjum AA 2022. Evaluating the effectiveness of multidrug resistant Staphylococcus aureus mastitis vaccines in dairy cattle. Pak Vet J, 42(3): 300-307.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)