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Therapeutic Effect of Oroxylin A Against Bisphenol A-induced Kidney Damage in Rats: a Histological and Biochemical Study
Ayesha Ishtiaq1, Muhammad Umar Ijaz2, Nazia Ehsan2, Muhammad Imran3, Huma Naz4, Kaynat Alvi2 and Guoping Zhu1*

1Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China; 2Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan; 3Department of Parasitology, University of Agriculture, Faisalabad, Pakistan; 4Department of Zoology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan
*Corresponding author:


Bisphenol A (BPA) is a synthetic chemical compound used in plastic products that adversely affects health. BPA exposure is associated with multiple organ toxicity, particularly renal toxicity. Oroxylin A (ORA) is a flavonoid that has potential therapeutical properties. The current study explored the effect of different doses of ORA on BPA-prompted inflammatory responses and oxidative stress in kidneys. The experiment was executed on ninety-six rats (200 ± 20 g) that were categorized into 8 groups. The impact of orally treated various doses (15 mg/kg, 30 mg/kg and 60 mg/kg) of ORA was investigated against the toxic effects caused by 10 mg/kg of BPA. After 56 days of the experiment, rats were euthanized to detect the level of renal function markers (urea and creatinine), oxidative stress markers, inflammatory markers, antioxidant enzyme activity and histological architecture. According to the results, BPA administration decreased the glutathione level as well as activities of glutathione peroxidase, glutathione reductase, catalase, glutathione-S-transferase and superoxide dismutase, while increasing the level of the oxidative stress markers (malondialdehyde, reactive oxygen species and hydrogen peroxide). BPA exposure significantly increased the level of urea and creatinine, while decreasing creatinine clearance. Moreover, BPA exposure remarkably increased inflammatory responses by augmenting the activity of cyclooxygenase-2 and levels of nuclear factor kappa-B, tumor necrosis factor-α, interleukin-6 and interleukin-1β. Besides, histopathological damages were also observed in renal tissues followed by BPA exposure. Co-administration of ORA ameliorated the toxic effects of BPA on renal tissue in a dose-dependent manner. Thus, it is deduced that ORA might be used to counter the harmful effects of BPA on renal tissues.

To Cite This Article: Ishtiaq A, Ijaz MU, Ehsan N, Imran M, Naz H, Alvi K and Zhu G, 2022. Therapeutic effect of oroxylin A against bisphenol A-induced kidney damage in rats: a histological and biochemical study. Pak Vet J, 42(4): 511-516.  


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)