The present study was designed to investigate the impact of breeding methods like artificial insemination (AI) vs natural mating (NM) and body weight variations on bacterial contamination and antimicrobial resistance (AMR) in broiler breeders. Ross-308 broiler breeder hens (n=3000) and males (n=255) were selected for this study. Out of those 255 male birds 150 (10%) were selected for natural mating and 105 (7% of hens) for artificial insemination groups. Both male and female groups were further subdivided into three weight categories sub-groups; underweight (UW), standard weight (SW) and overweight (OW). Fifteen (3%) hens out of 500 hens from each weight group were randomly selected and slaughtered to get samples for bacteriological and molecular analysis. A total 18.89% and 35.92% samples were found positive for Salmonella and E. coli respectively. Results revealed that the bacteria like Salmonella pullorum-gallinarum and E. coli were found significantly higher in AI groups as compared to NM groups. It was also found that at late life cycle (60^th week of age), the presence of Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS), Salmonella pollurum-gallinarum, and E. coli were significantly higher than that of lower age groups. The results also indicated that there is a comparatively lesser chance of MG, MS, E. coli, and Salmonella pollurum-gallinarum at the 30^th week of age as compared to 45^th and 60^th weeks of age. On the basis of antibiotic sensitivity testing results; Oflaxacine and colistin were found susceptible among 23 tested antibiotics. In conclusion, it was observed that the chance of bacterial contamination increases with the increase of age in AI groups.