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Investigating the Bacteriological Contamination after Artificial Insemination in Early, Mid and Late Production Period in Broiler Breeder
Farhan Farooq1, Aayesha Riaz2, Zaib Ur Rehman1, Arfan Yousaf3, Javid Iqbal4, Tanveer Ahmad5 and Nasir Mukhtar5*

1Department of Poultry Sciences, PMAS Arid Agriculture University Rawalpindi Pakistan
2Department of Parasitology & Microbiology, PMAS Arid Agriculture University Rawalpindi Pakistan
3Department of Clinical Studies, PMAS Arid Agriculture University Rawalpindi Pakistan
4University of Agriculture, Faisalabad, Pakistan (TTS Campus)
5Department of Livestock Production and Management, PMAS Arid Agriculture University Rawalpindi Pakistan
*Corresponding author:;


The present study was designed to investigate the impact of breeding methods like artificial insemination (AI) vs natural mating (NM) and body weight variations on bacterial contamination and antimicrobial resistance (AMR) in broiler breeders. Ross-308 broiler breeder hens (n=3000) and males (n=255) were selected for this study. Out of those 255 male birds 150 (10%) were selected for natural mating and 105 (7% of hens) for artificial insemination groups. Both male and female groups were further subdivided into three weight categories sub-groups; underweight (UW), standard weight (SW) and overweight (OW). Fifteen (3%) hens out of 500 hens from each weight group were randomly selected and slaughtered to get samples for bacteriological and molecular analysis. A total 18.89% and 35.92% samples were found positive for Salmonella and E. coli respectively. Results revealed that the bacteria like Salmonella pullorum-gallinarum and E. coli were found significantly higher in AI groups as compared to NM groups. It was also found that at late life cycle (60th week of age), the presence of Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS), Salmonella pollurum-gallinarum, and E. coli were significantly higher than that of lower age groups. The results also indicated that there is a comparatively lesser chance of MG, MS, E. coli, and Salmonella pollurum-gallinarum at the 30th week of age as compared to 45th and 60th weeks of age. On the basis of antibiotic sensitivity testing results; Oflaxacine and colistin were found susceptible among 23 tested antibiotics. In conclusion, it was observed that the chance of bacterial contamination increases with the increase of age in AI groups.

To Cite This Article: Farooq F, Riaz A, Rehman ZU, Yousaf A, Iqbal J, Ahmad T and Mukhtar N, 2022. Investigating the bacteriological contamination after artificial insemination in early, mid and late production period in broiler breeder. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)