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Biological Activity of the Secondary Compounds of Guazuma ulmifolia Leaves to Inhibit the Eggs Haemonchus contortus Hatching
J Velázquez-Antunez1, J Olivares-Perez*1, A Olmedo-Juárez*2, S Rojas-Hernandez1, A Villa-Mancera3, T Romero-Rosales1, Zamilpa A4 and Gonzalez-Cortazar M4

1Maestría en Ciencias Agropecuarias y Gestión Local, Universidad Autónoma de Guerrero, Iguala, México
2Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Morelos, México
3Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, México. Centro de Investigaciones Biomédicas del Sur, IMSS, Xochitepec, Morelos, México.
*Corresponding authors:,


Epidemiologically Haemonchus contortus is the most important parasite in the tropics, females can produce up to ten thousand eggs and blood feed. As objective, it was evaluated the in vitro effect of the secondary compounds of Guazuma ulmifolia leaves extracted with hydroalcoholic (HA) solvent and by fractionation in liquid-liquid layer with ethyl acetate, to inhibit eggs hatching of H. contortus, to interrupt the biological cycle of the parasite. The bioactive compounds were identified by high performance liquid chromatography. Challenged doses were in the HA extract 1.25-40 mg/mL, and aqueous and organic fractions of 1.25-10 mg/mL, respectively, distilled water and 2% methanol were negative controls and thiabendazole (0.1 mg/mL) positive control, for a total of seventeen treatments. The treatments were tested in three replicates with four repetitions, the data were analyzed with a GLM in a completely randomized design using ANOVA to an alpha ≤0.05. The effective concentrations (EC50 and EC90) were estimated by Probit analysis in the SAS program. The secondary compounds identified were of the phenols group such as flavonols, flavones, coumaroyl derivatives and hydroxycinnamic acid. The HA extract and aqueous fraction showed a similar effect to the positive control from doses of 2.5 mg/mL and the organic fraction from 1.5 mg/mL (P<0.01). In HA extract and aqueous fraction, the EC50 and EC90 were similar, but in the organic fraction the ovicidal effect was at lower EC50 (0.86 mg/mL) and EC90 (1.67 mg/mL). In conclusion G. ulmifolia leaves contain secondary compounds able for interrupting the cycle of H. contortus in the egg stage and the content of phenols like hydroxycinnamic acid and coumaroyl derivatives could be responsible for the ovicidal effect.

To Cite This Article: Velázquez-Antunez J, Olivares-Perez J, Olmedo-Juárez A, Rojas-Hernandez S, Villa-Mancera A, Romero-Rosales T, Zamilpa A, Gonzalez-Cortazar M, 2022. Biological activity of the secondary compounds of Guazuma ulmifolia leaves to inhibit the eggs Haemonchus contortus hatching. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)