Comparative Transcriptomic Analysis of Spermatozoa from Xiangxi and
Simmental Bulls under Heat Stress: Implications for Fertility
Prediction
Jianbo Li 1,2, Xin Wang 3,#, Ao Sun 1,
Haobang Li 1, Yang Luo 1, Fang He 1,
Cheng Huan 1, Xu Zhou 3, Chunjin Li 3,*,
Baizhong Zhang 1,2 and Kangle Yi 1,2,*
Jianbo Li 1,2, Xin Wang 3,#, Ao Sun 1,
Haobang Li 1, Yang Luo 1, Fang He 1,
Cheng Huan 1, Xu Zhou 3, Chunjin Li 3,*,
Baizhong Zhang 1,2 and Kangle Yi 1,2,* #Contributed
equally with the first author
*Corresponding author:yikangle@yeah.net
and llcjj158@163.com
Abstract
In the current study, a total of six 5-year-old healthy bulls reared in heat
stress zone were used and
semen from these bulls was collected by artificial vagina and undergoes for
semen quality analysis. Results of semen quality parameters showed that the
average semen volume and sperm density of Simmental bulls were 9.3mL and 1.23 ×
109 mL, and sperm acrosome integrity rate were lower as compared to
Xiangxi cattle bulls. The transcriptome analysis revealed a 36.81Gb clean data
and the obtained clean data of each sample was reached 5.84Gb. The transcriptome
analysis further revealed that the percentage of q30 base was 91.19% or above.
Based on the comparison results, 4,890 new genes were found, including 2,451
functional annotations. Go analysis explored that GO annotation system included
3 main sections that were biological processes, molecular functions and cellular
components, respectively. In conclusion, results showed that the average semen
volume and sperm density of Simmental bulls was higher and sperm motility and
sperm acrosome integrity rate was lower as compared to Xiangxi cattle bulls.
This study has developed a transcriptomic profile of spermatozoa of Xiangxi
cattle and Simmental cattle bulls and reported Xiangxi cattle and Simmental
cattle bull’s spermatozoa change the gene expression involved in various
biological process. This study could provide the experimental basis of poor
fertility in Xiangxi cattle bulls and provide possible fertility biomarkers.
To Cite This Article:
Li J, Wang X, Sun A, Li H, Luo Y, He F, Huan C, Zhou X, Li C, Zhang B, Yi K,
2022. Comparative transcriptomic
analysis of spermatozoa from Xiangxi and Simmental bulls under heat stress:
implications for fertility prediction. Pak Vet J.
http://dx.doi.org/10.29261/pakvetj/2022.083