Biosynthesis and Evaluation of
Cinnamomum zeylanicum
Nanomaterials for the Treatment of Polycystic Ovary Syndrome in Mice
Liaqat I1*, Noor S1, Qureshi AS2,
Ali S1, Liaqat I1, Al-Arifa N3,
Alam S1, Ajmal A1, Zia T1 and
Munawar M1
1Department
of Zoology, Government College University Lahore, Pakistan
2Department
of Anatomy, Faculty of Veterinary Science, Faisalabad, Pakistan
3Department
of Zoology, Lahore College for Women University Lahore, Pakistan
*Corresponding author:
Irfana.liaqat@gcu.edu.pk
Abstract
Polycystic ovary syndrome (PCOS) or Stein Leventhal
Syndrome is an inflammatory metabolic disorder resulting in ovarian
dysregulation and dysfunction leading to infertility in 7-20% of women globally.
Present experimental study was designed to evaluate the therapeutic effects of
medicinal plant (Cinnamomum zeylanicum)
using chitosan nanoparticles
on the hormonal profile and ovaries of PCOS-induced mice. Methanolic extract of
Cinnamomum zeylanicum was used to synthesize chitosan nanoparticles (CNPs) by
ionic gelatin method. The synthesized particles were characterized by FTIR and
XRD analysis. Experimental mice were given an intraperitoneal injection of 2
mg/Kg Estradiol valerate in 0.1 ml
corn oil to induce PCOS. Results have demonstrated a protective effect of
biomolecule-coated chitosan nanoparticles (CNPs) on the improved ovulatory
function of PCOS-induced mice. Mice treated with CNPs showed the recovery of the
estrous cycle and gonadotropin (FSH, LH) hormones. The FSH titre regain in
CZCNPH from 0.58 to 0.75 mIU/ml, similarly, LH levels were reduced from 0.65 to
0.53 mIU/ml. The biomolecules loaded CNPs showed the formation of the oocyte,
the formation of zona pellucida, zona granulosa and the dissolution of cysts in
PCOS mice. Histomor[hometric analysis showed high diameter of oocytes in CZCNPH
group. We conclude that the biomolecules loaded CNPs can effectively be used to
treat PCOS as compared to synthetic drugs.
To Cite This Article:
Liaqat I, Noor S, Qureshi AS, Ali S, Liaqat I, Al-Arifa N, Alam S, Ajmal A, Zia
T and Munawar M,
xxxx. Biosynthesis and evaluation of
Cinnamomum zeylanicum nanomaterials for the treatment of polycystic ovary
syndrome in mice. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2023.004