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Purification, Characterization and Protective Effects of Indigenous Yeast Derived β-glucans against Salmonellosis in Broilers
Muhammad Irfan Anwar1*, Muhammad Ali Tahir1, Mian Muhammad Awais1, Ahmad Raza1,2, Masood Akhtar1, Faqir Muhammad2, Muhammad Raza Hameed1 and Nabeel Ijaz3
1Department of Pathobiology, Faculty of Veterinary Sciences, Bahauddin Zakariya University Multan Pakistan
2Department of Biosciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University Multan Pakistan
3Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University Multan Pakistan
*Corresponding author:;


Indigenous Saccharomyces (S.) cerevisiae was cultured under submerged fermentation conditions and β-glucans were extracted by enzymatic method. The extracted β-glucans were characterized by FT-IR and their spectra presented peaks at a wavelength of 1021 cm-1. Safety and mutagenicity testing of β-glucans showed them non-mutagenic and non-toxic up to a concentration of 200 mg/mL. For immunotherapeutic effects against Salmonella (Sal.) typhimurium infection, broiler chicks (n=120) were randomly divided into 4 equal groups viz. A (negative control), B (positive control challenged with Sal. typhimurium; 1.0× 1010 cfu), C (β-glucans at the dose rate of 50 mg/kg feed + Sal. typhimurium challenge), and D (β-glucans at the dose rate of 100 mg/kg feed + Sal. typhimurium challenge). Results revealed protective effects of β-glucans against Sal. typhimurium infection as depicted by mild clinical signs along with minimal/no gross and histopathological lesions. Immunological responses were also higher (P<0.05) in β-glucans administered birds. β-glucans also modulated intestinal morphology with improved villi-height and crypt-depth ratio. In conclusion, β-glucans isolated from indigenous S. cerevisiae conferred protective effects against salmonellosis and therefore can be used successfully in broilers.

To Cite This Article: Anwar MI, Tahir MA, Awais MM, Raza A, Akhtar M, Muhammad F, Hameed MR and Ijaz N, 2023. Purification, characterization and protective effects of indigenous yeast derived β-glucans against salmonellosis in broilers. Pak Vet J, 43(2): 297-302.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)