previous page   Pak Vet J, 2023, 43(2): 371-375   next page
Hyperbaric Oxygen Therapy, a Possible New Approach in the Treatment of Retinal Damage in Methanol Poisoning in Rats
Mehmet Akif Baktir1,7, Pınar Alisan Suna2, Aydin Alan3, Arzu Yay2,4, Aysun Cetin5 and Ahmet Cumaoglu6
1Erciyes University, Faculty of Medicine, Department of Physiology. Turkey; 2Erciyes University, Faculty of Medicine, Department of Histology. Turkey; 3Erciyes University, Faculty of Veterinary, Department of Anatomy. Turkey; 4Genome and Stem Cell Center (GENKOK), Erciyes University, Kayseri, Turkey; 5Erciyes University, Faculty of Medicine, Department of Biochemistry. Turkey; 6Erciyes University, Faculty of Pharmacy, Department of Biochemistry. Turkey; 7Experimental and Clinical Research Center (DEKAM), Erciyes University, Kayseri, Turkey
*Corresponding author:


It has been established that peroxidation, inflammatory processes, and organ damage are involved in the pathogenesis of methanol intoxication. Research has shown that using hyperbaric oxygen reduces oxidative stress, inflammation, and tissue damage. In this research, it was investigated how hyperbaric oxygen therapy affected methanol-induced peroxidation and retinal damage in rats. The animals were classified into six categories (n = 8): control group (C), nitrous oxide (N2O) treated group, hyperbaric oxygen (HBO) treated group, methanol (MeOH) treated group, N2O+methanol treated group (N2O+MeOH) and N2O+methanol+hyperbaric oxygen (N2O+MeOH+HBO) treated group. Folate deficiency was started with N2O, four hours before the starting dose (4g/kg) of methanol. The rats were given a methanol maintenance dose (2g/kg, i.p) 24h and 48h after the starting dose. After each methanol administration, rats were given HBO (2 ATA) for 1 hour. Methanol intoxication decreased plasma folic acid levels while increasing formate levels and oxidative stress index and it caused a decrease in the amount of the retinal ganglion cells and thinned the total retinal thickness in folate-deficient mice. HBO treatment reduced the oxidative stress index but did not adequately improve retinal healing. Our findings indicate that the HBO treatment used to treat retinal damage in methanol poisoning was ineffective. However, increasing the duration of HBO therapy may be effective in preventing methanol-induced retinal damage.

To Cite This Article: Baktir MA, Suna PA, Alan A, Yay A, Cetin A, Cumaoglu A, 2023. Hyperbaric oxygen therapy, a possible new approach in the treatment of retinal damage in methanol poisoning in rats. Pak Vet J, 43(2): 371-375.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)