Mycotoxins are secondary metabolites produced by pathogenic fungi under suitable environmental conditions. Moringa oleifera and vitamin E are known antioxidants and immunostimulants. In present experimental study, M. oleifera and vitamin E were administered in birds feed to mitigate the toxico-pathological impacts of aflatoxin B1 in white Leghorn layer males. A total of 120 white Leghorn layer male birds were equally divided into six experimental groups. Group A was kept as negative control, group B was fed 400ppb of aflatoxin, while D and F groups were fed normal feed and supplemented with vitamin E (100ppm) and 1 %M. oleifera, respectively. The birds in C and E groups were given 400 ppb AFB1 in feed and supplemented with vitamin E and M. oleifera, respectively. During the 60 days of experimental trial, hematobiochemical and histopathological parameters were studied. The data obtained was analysed by analysis of variance test and group means were compared by DMR test using M STAT C statistical software. During and at the end of experimental trial, male layer birds were slaughtered, collected kidney and liver were fixed in 10% neutral buffered formalin to investigate gross and histopathological alternations. There were significant alterations in hematological and serum biochemical parameters of aflatoxin treated birds. Serum enzymes including ALT, AST and LDH values were significantly increased in group B in contrast to control group A. The histopathological changes in hepatic and renal parenchyma induced by AFB1 were prominent and no such changes were seen in A, D and F groups, while C and E group showed mild necrotic changes which was an indication of ameliorative effects of vitamin E and M. oleifera. It may be concluded that vitamin E and M. oleifera have potential to mitigate toxicity of aflatoxin B1.