The presence of pathogenic Escherichia coli in human consumption of food fish can cause disease and might increase the likelihood of antibiotic resistance transmission from aquatic bacteria to bacteria that infect humans. The study aimed to detect the copper resistance gene (copA) in the isolated E. coli. from the common carp fish of Dukan Lake in Sulaymaniyah province. Ninety fish samples were taken from three fishing places at Dukan Lake; the center of Dukan Lake, Ranya, and Qaladze. Isolation and identification of E. coli were performed using culturing and molecular methods. Of the 90 fish samples, 58 (64.4%) were positive for E. coli. The isolates were screened for detection of the 16S rRNA and the copA genes by Polymerase Chain Reaction (PCR) using specific primers. Of the 90 fish samples, 46 (51.1%) were positive for the 16S rRNA gene, and 6 (6.7%) were positive for the copA gene. The E. coli gene 16S rRNA has been sequenced and assigned accession number ON385541 under the name of E. coli isolate 2022 mabao in NCBI GenBank. The phylogenetic analysis of 16S rRNA gene sequences revealed that E. coli strains were related to other E. coli bacteria from other countries, with the maximum identity being 99.61 percent. We conclude that small amounts of E. coli found in common carp in three distinct areas of Dukan Lake have the copA gene, and an investigation of the 16S rRNA gene sequence in our study revealed that this gene's conserved region only had a few small nucleotide alterations.