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Investigations of Hemato-Biochemical, Histopathological, Oxidative Stress and Reproductive Effects of Thiram in Albino Rats

Ramya Ahmad Sindi1, Sana Alam2, Muhammad Rizwan2, Muhammad Irfan Ullah3, Nabeel Ijaz4, Zahid Iqbal5, Rabia Muzafar2, Rabia Akram6, Muhammad Waqas Nazar7 and Riaz Hussain7*

1Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, Saudi Arabia
2Department of Zoology, Islamia University of Bahawalpur-63100, Pakistan
3Department of Pathobiology, Faculty Veterinary Sciences, Bhauddin Zakariya University, Multan, Pakistan
4Department of Clinical Sciences, Faculty Veterinary Sciences, Bhauddin Zakariya University, Multan, Pakistan
5Department of Pharmacology and Toxicology, Faculty of Veterinary and Animal Sciences, Islamia University of Bahawalpur-63100, Pakistan
6Institute of Pure and Applied Biology, Zoology Division, Bhauddin Zakariya University, Multan, Pakistan
7Department of Pathology, Faculty of Veterinary and Animal Sciences, Islamia University of Bahawalpur-63100, Pakistan
*Corresponding author:


Thiram, being a dithiocarbamate fungicide, is frequently used to protect a variety of food crops (vegetables, ornamentals and fruits) and seeds and it may remain in various products of plants, water and in soil after applications. The current study was executed to record the toxic effects of thiram in male albino rats. A total of 15 adult male albino rats were obtained from local market and were placed in wire cages in three groups (A-C). Thiram was administered daily to all rats of group B (50 mg/kg bw) and C (75 mg/kg bw) for 21 days and different tissues (blood, testes and liver) were harvested for determination of hematological, reproductive and oxidative stress parameters. Results revealed significantly lower values of red blood cell counts and hemoglobin quantity while increased values of total white blood cell counts and neutrophil percentage. The different serum biomarkers including alkaline phosphatase, alanine aminotransferase, urea, creatinine, triglyceride, glucose and cholesterol significantly escalated in thiram treated rats. Results showed significantly lower levels of antioxidant enzymes (peroxidase and catalase) while higher values of oxidative stress biomarkers such as reactive oxygen species (ROS) and thiobarbituric acid substance (TBARS) in liver of treated albino rats. Histopathological investigations indicated different pathological lesions including degeneration and damage of spermatogonia, cellular debris in lumen of seminiferous tubules, necrosis of spermatids, decrease in frequency of seminiferous tubules with normal spermatozoa, arrest of process of spermatogenesis and inflammatory materials in testes of rats. This work shows that thiram causes disruption of normal functions of blood-biochemistry, antioxidant enzymes and reproductive tissues through induction of oxidative stress..

To Cite This Article: Sindi RA, Alam S, Rizwan M, Ullah MI, Ijaz N, Iqbal Z, Muzafar R, Akram R, Nazar MW and Hussain R, 2023. Investigations of hemato-biochemical, histopathological, oxidative stress and reproductive effects of thiram in albino rats. Pak Vet J, 43(2): 255-261.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)