PAKISTAN VETERINARY JOURNAL

Pak Vet J, 2023, 43(3): 456-462

Complete Genome of Multi-Drug Resistant Staphylococcus Aureus in Bovine Mastitic Milk in Anhui, China

Junjun Liu¹, Xinglin Zhang², Jianrui Niu², Zhaoqing Han², Chongliang Bi², Khalid Mehmood³, Dunia A Al Farraj⁴, Inshad Alzaidi⁴, Rashid Iqbal⁵ and Jianhua Qin¹*

¹College of Veterinary Medicine/Traditional Chinese Veterinary Medicine, Hebei Agriculture University, Baoding 071001, People’s Republic of China; ²College of Agriculture and Forestry, Linyi University, Shuangling Road, Linyi City, Shandong, 276005, PR China; ³Department of Clinical Medicine and Surgery, Faculty of Veterinary & Animal Sciences, The Islamia University of Bahawalpur 63100, Bahawalpur, Pakistan; ⁴Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia; ⁵Department of Agronomy, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur 63100, Bahawalpur, Pakistan
*Corresponding author: qjhqqq@126.com

Abstract

Staphylococcus aureus is a critical pathogen causing serious mastitis with huge economic losses in bovines. To reveal drug-resistant genes, a complete genome sequence of a multi-drug-resistant bacterium S. aureus derived from bovine mastitic milk was performed via High-throughput sequencing. Results revealed that the genome length of current S. aureus was 2.85 Mbp consisting of 2656 coding sequence genes, 60 tRNA, 19 rRNA, and 5 genomic islands. Functional annotation of S. aureus encoded proteins showed that 2090 (COG), 1586 (KEGG), 1600 (GO), 2648 (Refseq), 2300 (Pfam), 964 (SwissProt), and 1826 (TIGRFAMs) proteins were annotated. Antibiotic-resistant genes of tet, mepR, mepA, mgrA, norA, blaZ, arlS, arlR and LmrS in S. aureus genome were found via CARD annotation. More than 60 bacterial virulence factors including commonly known clfA, hla, hlb, fnbB, fnbA in S. aureus genome were uncovered through VFDB annotation. The current study may contribute towards effective treatment and developing new antibiotics against mastitis caused by S. aureus.

To Cite This Article: Liu J, Zhang X, Niu J, Han Z, Bi C, Mehmood K, Farraj DAA, Alzaidi I, Iqbal R and Qin J, 2023. Complete genome of multi-drug resistant Staphylococcus Aureus in bovine mastitic milk in Anhui, China. Pak Vet J, 43(3): 456-462. http://dx.doi.org/10.29261/pakvetj/2023.052

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)