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Calcium Nanoparticles Induce Oxidative Stress in Erythrocytes, Neurotoxicity and Testicular Toxicity in Albino Rats (Rattus norvegicus)

Ahsan Ullah1, Fatimah A. Al-Saeed2, Alduwish Manal Abduallah3, Ahmed Ezzat Ahmed4,5, Asim Shahzad1, Nouman Amjad1, Arooj Ali6, Mohamed S. Mostafa7 and Riaz Hussain1*

1Department of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan; 2Biology Department, College of Science, King Khalid University, Saudi Arabia; 3Department of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia; 4Biology Department, College of Science, King Khalid University, 61413 Abha, Saudi Arabia; 5Theriogenology Department, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, Egypt; 6Institute of Physics, Faculty of Physical & Mathematical Sciences, The Islamia University of Bahawalpur, 63100, Pakistan; 7Department of Chemistry, Faculty of Science, Jazan University P.O. Box 45142, Saudi Arabia
*Corresponding author:


Calcium nanoparticles (Ca-NPs) are emerging as promising materials and are frequently used in different areas of medical science including treatment therapy. In current study, different unwanted impacts of Ca-NPs were observed on various tissues including teeth, testes, brain and erythrocytes of male albino rats. A total of twelve active and sexually mature albino rats free from any obvious clinical ailments were procured from local market and were placed in wire cages in three groups. The rats in groups B and C were administered Ca-NPs at two dosage levels (25 and 50 mg/kg body weight) while the rats in control group received no treatments. No physical changes in terms of mortality, morbidity, behavioral and neurological signs were observed in rats of all groups. Different pathophysiological alterations such as significantly increased contents of oxidative parameters (reactive oxygen species and thiobarbituric acid reactive substance) while reduced contents of antioxidant enzymes (reduced glutathione, peroxidase, catalase and superoxide) in red blood cells (RBC) of albino rats of group B and group C were recorded. Results on various histo-architecture changes in different visceral organs like the testes, brain, and teeth exhibited various microscopic alterations in albino rats treated with high dosages of Ca-NPs as compared to the control group. Therefore, the current study showed that the prepared Ca-NPs had adverse physiological effects on different tissues in the albino rats.

To Cite This Article: Ullah A, Al-Saeed FA, Abduallah AM, Ahmed AE, Shahzad A, Amjad N, Ali A, Mostafa MS and Hussain R, 2023. Calcium nanoparticles induce oxidative stress in erythrocytes, neurotoxicity and testicular toxicity in albino rats (Rattus norvegicus). Pak Vet J, 43(2): 241-247.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)