This study aims to determinate in vitro anthelmintic activity of the plant extracts from the aerial part of five different Artemisia L. species (A. absinthium L., A. abrotanum L., A. annua L., A. incana (L.) Druce, A. tournefortiana Rchb.) growing in Türkiye. Depending on the ethnobotanical usage, the aqueous plant extracts were prepared by decoction and maceration methods. The anthelmintic activity of the plant extracts on Haemonchus (H.) contortus eggs and larvae was assessed using in vitro test methods. The extracts were prepared in five concentrations (50; 25; 12.5; 6,25; 3.125 and 1.5625 mg/mL) by using PBS for egg hatch and larval motility inhibition assay. In comparison to the negative control group, the extracts had significant anthelmintic effect on H. contortus eggs and larvae (p<0.05). In the egg hatch assay, all plants were found to be fully effective in the concentration range studied. In the analysis of larval motility, the activity was correlated with the quantities of the plant extracts. When the concentration-activity values of the plants were examined for both extraction methods, the highest activity was in A. annua and the lowest activity was observed in A. tournefortiana. Due to the development of resistance to anthelmintic drugs, natural sources such as Artemisia species can be considered as an alternative for the treatment of nematodes.