Comparative Efficacy of GnRH, HCG and Seminal Plasma for
Induction of Ovulation During the Breeding Season in the Dromedary
Camel (Camelus dromedarius)
Danish Aziz1, Zafar Iqbal Qureshi1,2*,
Muhammad Salman Waqas1, Misbah Ijaz3, Imtiaz
Hussain4 and Muhammad Wasim Usmani2
1Department
of Theriogenology, Faculty of Veterinary Science, University of
Agriculture, Faisalabad, Pakistan; 2Faculty of
Veterinary and Animal Sciences, Ziauddin University, Karachi,
Pakistan; 3Department
of Clinical Medicine and Surgery, Faculty of Veterinary Science,
University of Agriculture, Faisalabad, Pakistan; 4Camel
Breeding and Research Station, Rakh Mahni, Bhakar, Livestock and
Dairy Development, Punjab, Pakistan.
*Corresponding author:
zafar.qureshi@zu.edu.pk
Abstract
In the current study,
efficacy of GnRH, hCG and seminal plasma for the induction of ovulation in
dromedary camels during breeding season was investigated. Sixteen female
dromedary camels, having ovarian follicle size of 12-19mm, were randomly divided
into four equal groups G, H, SP and C. The camels in groups G and H were
injected with 2ml (50μg) GnRH analogue and
3000IU of hCG, respectively. Camels in group SP were injected with 1ml seminal
plasma mixed with 1ml normal saline and group C (control group) was injected
with 1ml normal saline intramuscularly. For
the confirmation of ovulation, and CL development, ovaries of experimental
camels were examined through trans-rectal ultrasonography on days 1, 2, 3, 8 and
11 post-treatment. The Progesterone ELISA
kit was used to determine serum progesterone (P4) concentrations in samples
collected on days 0, 3, 6, 8, and 11 of treatment.
The results revealed that ovulation rates were 75, 50, 50 and 0% in groups G, H,
SP and C, respectively, values for G, H and SP groups were higher compared to
group C (p<0.05), while former three groups differed non-significantly from one
another. Serum P4 concentrations were <0.5ng/ml in camels of all groups
on day 0. However, among ovulated camels, the serum P4 concentration was highest
on day 8, when it was 4.80±0.15, 4.15±0.14 and 2.67±0.15ng/ml in SP, H and G
groups, respectively; the difference among 3 groups was significant (p<0.05). It
was concluded that in dromedary females, use of hormones for the induction of
ovulations is an effective option and can safely be used in camel breeding
programs, as hormones are easily available compared to seminal plasma.
To Cite This Article:
Aziz D, Qureshi ZI, Waqas MS, Ijaz M, Hussain I
and Usmani MW, 2023. Comparative efficacy of GnRH, HCG and seminal plasma for induction
of ovulation during the breeding season in the dromedary camel(Camelus dromedarius).
Pak Vet J, 43(4): 809-813. http://dx.doi.org/10.29261/pakvetj/2023.095