Comparative Analysis of Nasal Microbial Community
between Tibetan Sheep with different Ages
Xin Ding1,2#, Huyan Jiang3#,
Ranran Zhang1,2, Xin Chen1, Han Yu1,
Yuan Zu1, Shuaishuai Tan1, Xin Wang1,
Qi Wang1, Wen Xu1, Dalia Fouad4,
Muhammad Usman Saleem5 and Zhigang Liu1,2*
1College
of Life Science, Anqing Normal University, Anqing 246011, China;
2Research Center of Aquatic Organism Conservation and
Water Ecosystem Restoration in Anhui Province, Anqing Normal
University, Anqing 246011, China;
3Department
of Chemistry, Anqing Normal University, Anqing 246011, China;
4Department
of Zoology, College of Science, King Saud University, PO Box 22452,
Riyadh 11495, Saudi Arabia; 5Department of Biosciences,
Faculty of Veterinary Sciences, Bahauddin Zakariya University,
Multan, Pakistan *Corresponding
author:
lzg2881@126.com
(Zhigang
Liu)
Abstract
Tibetan sheep is an important domestic animal inhabiting the Tibetan plateau,
which is closely related to local economic development and national culture.
Presently, there are many studies on the gut microbiota of Tibetan sheep, but
little is known about the nasal microbial community. Here, we investigated the
distributions and changes of the nasal microbial community in Tibetan sheep at
different ages. Results of amplicon sequencing showed that 476,275 and 484,669
effective sequences were generated in the ASG (adult Tibetan sheep) and YSG
(young Tibetan sheep) groups, respectively. These sequences were clustered into
6,817 OTUs and the ASG group and YSG group have 2,133 and 5,176 OTUs,
respectively. Results of alpha diversity indicated that the ASG group had a
significantly higher Chao1 index than the YSG, whereas Simpson and Shannon
indices had no significant difference. Metastatistical analysis showed that
compared with the ASG group, 8 bacterial phyla were significantly increased and
2 bacterial phyla were significantly decreased in the YSG group. At the genus
level, 185 bacterial genera were significantly increased and 25 bacterial genera
were significantly decreased in the YSG group compared with the ASG group. In
conclusion, this study provided a preliminary analysis of the dynamic changes
and distribution of nasal microbial
community in Tibetan sheep.
Results showed that the abundance and composition of the nasal microbial
community of Tibetan sheep were significantly different at different ages.
To Cite This Article:
Ding X, Jiang H, Zhang R, Chen X, Yu H, Zu Y, Tan
S, Wang X, Wang Q, Xu W,
Fouad D, Saleem MU and Liu Z, 2023. Comparative analysis of nasal
microbial community between tibetan sheep with different ages. Pak Vet J, 43(4): 723-731. http://dx.doi.org/10.29261/pakvetj/2023.075