Peripheral nerve injury (PNI) is among the leading health issues affecting the modern era. Currently, there are no effective therapeutic strategies to heal nerve damage and ensure fully functional recovery. Probiotics can serve as an appealing and effective option to close this gap via the gut microbiota. The purpose of the study was to evaluate the role of probiotics on functional recovery after nerve injury. For this purpose, sixty healthy BALB/c mice were divided into 04-groups. The control group was given a routine diet. In contrast, positive control, pre-injury probiotics and post-injury probiotics were administrated their respective treatments orally from the day of nerve injury to the end of the project. The sciatic functional index, grip strength, pinprick, and hot plate tests were used to analyse the retrieval of motor and sensory functions, and the results for the pre-injury probiotics group were highly significant. Additionally, the fiber count and surface area of the Tibialis anterior muscle were significantly improved in this group. When compared to the control and post-injury probiotics groups, this group's much lower total oxidant status and increased total antioxidant capacity indicate that probiotics have a strong potential to improve the restoration of muscle function when introduced before the injury. These results imply that probiotics are able to accelerate functional recovery following a peripheral nerve injury via the gut-brain axis. Nonetheless, future studies are warranted to identify the underlying mechanism of probiotics that boosts functional restoration.