The present study was designed to investigate the prevalence, epizootological profile, and haematological and serum biochemistry changes in coccidiosis infected pet dogs of Rawalpindi-Islamabad, Pakistan. For this purpose, 150 blood and faecal samples were collected from dogs of various breeds. Data regarding the epizootolgical risk factors were collected through a questionnaire at the time of sample collection. The faecal examination illustrated a 16.67% prevalence of coccidiosis in dogs. Data analysis of associated risk factors revealed that locality (P = 0.001), domestication (P = 0.000), and sanitary conditions (P = 0.000) have a significant impact. Whereas there was a non-significant association among age, gender, breed and faecal consistency in coccidiosis infected animals. Various hematological parameters were compared and revealed that non-significant difference in coccidiosis positive samples compared to the control group. The biochemical parameters showed significant differences in serum total protein (P = 0.001) and serum albumin (P = 0.003) among infected and control animal samples. This study finding provide baseline data and valuable information to the owners, public health authorities, researchers and the veterinary community on the risk factors, complications and probable transmission of canine coccidiosis.