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Discovery of the Enrichment Pathways and Biomarkers using Metabolomics Techniques in Unilateral and Bilateral Castration in Yellow Cattle
Ali Mujtaba Shah1,2, Zhisheng Wang1*, Rui Hu1, Quanhui Peng1, Huwai Zou, Lizhi Wang1 and Bai Xue1

1Low Carbon Breeding Cattle and Safety Production University Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, PR China; 2Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
*Corresponding author:


In the present study, the effect of unilateral (ULC) and bilateral castration (BLC) on metabolomics in yellow cattle was conducted. The current trial was comprised of 120 days of feeding following castration, after completion of the trial the blood samples were collected from all groups for metabolomics analysis. The results displayed 46 important metabolites were found when BLC compared to the CON group (VIP >1P < 0.05), such as GPCho (22:6/18:2), 7,8-Dihydrobiopterin, phosphocholine, phosphatidylcholine (PC), Butyryl-L-carnitine and 5-Hydroxyindoleacetic acid etc. The KEGG pathway analysis showed that BLC affects the 9 metabolic pathways in the yellow cattle serum when compared with the CON group. Biomarker analysis showed that the metabolites PC(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/18:2(9Z,12Z)),PC(20:4(8Z,11Z,14Z,17Z)/P-18:0),PC(18:0/22:5(4E,7E,10E,13E,16E))[U],PC(17:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z))and butyryl-L-carnitine etc. were the serum biomarkers between BLC and CON groups. In addition, when we compared the differential metabolites between ULC and BLC groups, 58 differential metabolites were found. KEGG pathway analysis showed that the metabolome pathway between ULC and BLC cattle affects 12 pathways in the serum of the yellow cattle. The BLC group cattle had a higher serum concentration of phosphatidylcholine and LysoPC enriched in the Glycerophospholipid metabolism pathway and a higher serum concentration of PC(16:0/20:4(5Z,8Z,11Z,14Z)) enriched in the Arachidonic acid and Linoleic acid metabolisms pathway, respectively. The Phospholipid Metabolites were the biomarkers in the serum of BLC cattle. Therefore, Castration enhanced the synthesis metabolism of unsaturated fatty acids and increased concentrations of unsaturated fatty acids, including linoleic acid (C18:2n6c), linolenic acid (C18:3n3), arachidonic acid (C20:2), docosahexaenoic acid (C22:6n3), in the fat tissue of cattle.

To Cite This Article: Shah AM, Zhisheng W, Rui H, Peng Q, Zou H, Wang L and Xue B, 2024. Discovery of the enrichment pathways and biomarkers using metabolomics techniques in unilateral and bilateral castration in yellow cattle. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)

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