Porcine rotavirus is a significant pathogen that causes rotavirus sickness in both humans and animals. G9 rotavirus, in particular, is thought to be a new rotavirus that has lately spread over the world between humans and pigs. However, the understanding of the pathogenicity of G9 rotavirus is limited to date. In the current study, a G9 porcine rotavirus strain was isolated in the fecal samples of diarrheal piglets from a large-scale pig farm located in Guangxi, China. Then, the virus was identified by western blotting, and the production dynamics of the virus were confirmed by an indirect immunofluorescence test with a viral titer of 10^7.46 TCID₅₀/mL. Based on the VP7, VP4 and VP6 genes, phylogenetic analysis and sequencing data indicated that the GX9579 strain was of genotype G9P [23]I5. In addition, to explore the pathogenicity of the isolated GX9579 strain, animal tests were performed herein. Fecal viral shedding was detected, and fecal excretion was the highest at 10⁵ copies/µL 24h after the challenge and then gradually decreased. Histopathological test results suggested the intestinal villous arrangement of infected piglets became shortened, partially shed, and broken, intestinal villous epithelial cells shed into the intestinal lumen, and the villi propria lamina became hyperemic with severe bleeding and submucosal edema. Positive signals were seen throughout the intestinal villi's epithelial cells and the small intestine glands, according to immunohistochemical studies. These results provide reference data for further studies on the epidemiology and pathogenesis of G9 swine rotavirus.