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In vitro Anthelmintic Efficacy of Three Plant Extracts against Various Developmental Stages of Haemonchus contortus
Muhammad Abubakar1, Muhammad Oneeb1*, Muhammad Rashid2, Kamran Ashraf1, Ghazanfar Ali Chisti1, Furqan Awan1 and Noor-ul-Ain Sarwar3

1University of Veterinary & Animal sciences, Lahore, Pakistan
2Livestock Production Research Institute Bahadurnagar, Okara, Pakistan
3Livestock and Dairy Development Department, Punjab, Pakistan
*Corresponding author:


Gastrointestinal nematodes, including Haemonchus (H.) contortus, have a notable impact in terms of impaired production, high fatalities, and substantial economic losses, affecting small ruminants in tropical countries, including Pakistan. The irrational use of synthetic chemicals has fueled anthelmintic resistance, particularly in H. contortus, as a formidable challenge, prompting a search for alternative treatment strategies. This study evaluated the anthelmintic potential of leaf extracts of three forage plants, including Leucaena leucocephala, Moringa oleifera, and Acacia ampliceps, against various developmental stages of H. contortus. Phytochemical screening revealed the presence of secondary metabolites in the extracts. In vitro assays were performed to evaluate the anthelmintic efficacy of the extracts on the inhibition of egg hatching, larval paralysis, and adult worm motility following the guidelines of the World Association for the Advancement of Veterinary Parasitology, while Albendazole was used as a positive control and PBS as a negative control. The highest concentrations of tannins and flavonoids in Acacia ampliceps and phenolic compounds in Leucaena leucocephala were detected in the extracts as major secondary metabolites. All the tested extracts showed significant time- and dose-dependent responses to various stages of H. contortus. The ED50 values showed that the lowest dose of Leucaena leucocephala (0.285), Moringa oliefera (0.81), and Acacia ampliceps (0.245) inhibited egg hatching and larval and adult motility, respectively. Extracts of Leucaena leucocephala exhibited significant inhibition of egg hatching (70.3%), whereas Moringa oliefera showed maximum percent inhibition (66.6) of larval motility. These findings suggest that these plants, traditionally used by local healers in Pakistan, may possess antiparasitic properties, encouraging further studies on the relationship between phytochemicals, extraction methods, and in vivo trials to explore their anthelmintic efficacy.

To Cite This Article: Abubakar M, Oneeb M, Rashid M, Ashraf K, Chisti GA, Awan F and Sarwar NUA, 2024. In vitro anthelmintic efficacy of three plants extracts against various developmental stages of Haemonchus contortus. Pak Vet J.   


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)

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