Prevalence and Phylogenetic Analysis of
Mycoplasma hyopneumoniae
in Native Pigs from Shanghai, China
Dengke Zhong1#,
Jiayang Zheng2#,
Zhixin Guan2, Abdul Wahaab2, Junjie Zhang2,
Muhammad Naveed Anwar2,3* and
Jianchao Wei2*
1Shanghai
Vocational College of Agriculture and Forestry, Shanghai 201600, P.
R. China; 2Shanghai Veterinary Research Institute,
Chinese Academy of Agricultural Science, Shanghai, 200241, P.R.
China; 3Institute of Microbiology, University of
Agriculture, Faisalabad 38040, Pakistan
#
Equal contributors
*Corresponding author:
jianchaowei@shvri.ac.cn
(JW);
naveed.anwar@uaf.edu.pk
(MNA)
Abstract
Mycoplasma hyopneumoniae
(M.
hyo)
causes significant losses to the economy of the pig industry in the world.
Little information is available about the prevalence of
M. hyo
infection in Chinese native pigs. We report herein a high seroprevalence (61.5%)
to M. hyo in Chinese native pigs (Meishan
and Fengjing) from Shanghai, China. Meishan or Fengjing pigs had a significantly
higher seroprevalence compared to Duroc × Landrace × Yorkshire pigs (p <
0.05). Among Meishan and Fengjing groups, the peak prevalence was in breeding
pigs (74.5%), followed by suckling piglets (71.4%), finishing pigs (69.6%) and
nursery pigs (34.7%) and differences were statistically significant (p
<0.05). PCR analysis of the nasal swabs showed that 5.1% (5/98) of the Meishan
pigs (6.5%,3/46) or Fengjing pigs (3.8%,2/52) were positive for M. hyo.
The nucleotide sequences of p46 genes of
M. hyo from Meishan pigs or Fengjing pigs were highly conserved with
98.6-99.8% sequence homology with other reference strains. Our data confirm that
M. hyo
infection in
native
pigs was
highly prevalent
in Shanghai, and thus
integrated strategies should be taken to control the disease.
To Cite This Article:
Zhong D,
Zheng J,
Guan Z, Wahaab A, Zhang J, Anwar MN and
Wei J,
2024.
Prevalence and phylogenetic analysis of
Mycoplasma hyopneumoniae in native pigs from Shanghai, China.
Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2024.202