This study aimed to investigate the effects of platelet rich plasma (PRP) and streptokinase (SK) on postoperative intra-abdominal adhesion formation in rats. The 4-month-old, healthy, non-pregnant female Wistar-Albino rats were used as animal material. The remaining 24 animals were divided into three groups with 8 rats in each group (PRP, SK and Control). The fibroblast proliferation, mononuclear cell infiltration and giant cells were clearly prominent in control group. A decrease was observed in the ratio of these cells in the PRP and SK groups. The comparison of the expression levels of vWF, CASP3 and IL-6 genes showed that the vWF gene was downregulated in the SK group and the IL-6 gene was downregulated in the PRP and SK groups. They were statistically significantly compared to the control group. It was concluded that the expression level of CASP3 gene decreased in the PRP and SK groups compared to the control group, but this decrease was not statistically significant. The PRP and SK could be applied intraperitoneal to prevent the adhesions after intra-abdominal surgery.